| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PP17; TIP47; M6PRBP1 |
| Entrez GeneID | 10226 |
| clone | 6C9D9 |
| WB Predicted band size | 47kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PLIN3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PLIN3抗体的3-4条参考文献示例(文献为虚构，仅作格式参考)：
1. **文献名称**：*"Perilipin 3 Modulates Lipid Droplet Dynamics in Adipocytes"*
**作者**：Brasaemle DL, et al.
**摘要**：研究揭示了PLIN3在脂肪细胞脂滴形成和稳定性中的作用，表明其通过调控脂解酶活性影响脂质代谢，并验证了特异性抗体在定位PLIN3蛋白的应用。
2. **文献名称**：*"PLIN3 Expression in Hepatic Steatosis: Role in Lipid Storage and Inflammation"*
**作者**：Sztalryd C, et al.
**摘要**：通过免疫组化和Western blot分析，发现PLIN3在非酒精性脂肪肝中表达上调，提示其可能通过促进脂滴积累参与疾病进展。
3. **文献名称**：*"Antibody-based Characterization of PLIN3 in Macrophage Foam Cells"*
**作者**：Granneman JG, et al.
**摘要**：利用PLIN3特异性抗体，证实其在巨噬细胞泡沫化过程中与胆固醇酯储存相关，可能成为动脉粥样硬化的潜在标志物。
4. **文献名称**：*"PLIN3 Regulates Autophagy-Lipid Metabolism Crosstalk in Cancer Cells"*
**作者**：Kaushik S, et al.
**摘要**：研究通过免疫荧光和Co-IP实验，揭示PLIN3通过结合自噬相关蛋白(如LC3)调控肿瘤细胞脂质代谢，抗体验证了其亚细胞定位。
(注：以上文献及作者为示例，实际引用需查询真实数据库如PubMed。)
PLIN3 (Perilipin 3), also known as TIP47 (Tail-Interacting Protein 47 kDa), is a member of the perilipin family of lipid droplet-associated proteins. It plays a critical role in lipid metabolism by regulating the formation, stabilization, and intracellular trafficking of lipid droplets (LDs), which are dynamic organelles involved in lipid storage and mobilization. PLIN3 is ubiquitously expressed and interacts with LDs during their biogenesis, facilitating neutral lipid deposition and protecting LDs from premature hydrolysis by lipases. Its expression is modulated by metabolic cues, such as nutritional status and hormonal signals, linking it to energy homeostasis.
PLIN3 antibodies are essential tools for studying LD biology and metabolic disorders. These antibodies enable the detection, localization, and quantification of PLIN3 in cells and tissues through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use PLIN3-specific antibodies to investigate its role in pathologies such as obesity, non-alcoholic fatty liver disease (NAFLD), and atherosclerosis, where aberrant LD accumulation is a hallmark. Additionally, PLIN3 antibodies help explore its interaction with other LD-associated proteins (e.g., PLIN2. ATGL) and membrane trafficking machinery. Validated antibodies are critical for distinguishing PLIN3 from structurally related perilipin family members, ensuring specificity in experimental models. As interest in metabolic diseases grows, PLIN3 antibodies remain pivotal in elucidating molecular mechanisms underlying lipid dysregulation and identifying therapeutic targets.
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