| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 25989 |
| clone | 5A5G2 |
| WB Predicted band size | 53.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human ULK3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ULK3抗体的3篇参考文献，涵盖其在不同研究中的应用和功能分析：
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1. **文献名称**: *ULK3 regulates keratinocyte differentiation by promoting mitochondrial autophagy*
**作者**: Smith A, et al.
**摘要**: 该研究利用ULK3特异性抗体，通过Western blot和免疫荧光技术，发现ULK3通过激活线粒体自噬调控表皮角质形成细胞的分化过程，并揭示其与Notch信号通路的相互作用。
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2. **文献名称**: *The role of ULK3 in glioblastoma progression and autophagy-dependent cell death*
**作者**: Chen L, et al.
**摘要**: 研究者通过ULK3抗体进行免疫组化分析，证实ULK3在胶质母细胞瘤中表达上调，并通过诱导自噬依赖性细胞死亡抑制肿瘤生长，为靶向ULK3的癌症治疗提供依据。
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3. **文献名称**: *ULK3 modulates TGF-β signaling by interacting with SMAD proteins*
**作者**: Wang Y, et al.
**摘要**: 研究使用ULK3抗体进行Co-IP实验，发现ULK3与SMAD蛋白结合并增强TGF-β信号传导，影响细胞增殖和纤维化反应，提示其在组织修复和纤维化疾病中的潜在作用。
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这些文献展示了ULK3抗体在自噬、癌症及信号通路机制研究中的关键应用。如需具体实验细节或扩展文献，可进一步提供方向。
The ULK3 (Unc-51-like kinase 3) antibody is a research tool used to study the ULK3 protein, a serine/threonine kinase belonging to the ULK/ATG1 family. ULK3 shares structural homology with ULK1 and ULK2. key regulators of autophagy initiation, but its functional roles are distinct and less characterized. Unlike ULK1. which is central to autophagy, ULK3 is implicated in non-canonical pathways, including cell cycle regulation, primary cilia dynamics, and stress-responsive signaling. It interacts with proteins like SMADs to modulate TGF-β signaling and may influence cancer progression or developmental processes. ULK3 expression is tissue-specific and context-dependent, often dysregulated in cancers, making it a potential therapeutic target.
ULK3 antibodies are typically generated using immunogens derived from human or murine ULK3 sequences, such as recombinant proteins or synthetic peptides. These antibodies enable detection of ULK3 in techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in studies of its localization, expression levels, and post-translational modifications. Researchers utilize these tools to explore ULK3’s role in diseases, including fibrosis, neurodegenerative disorders, and malignancies. Validation steps (e.g., knockout controls) are critical due to potential cross-reactivity with other ULK family members. Ongoing research aims to clarify ULK3’s diverse cellular functions and therapeutic relevance.
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