| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | nan | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TCF-1 |
| Entrez GeneID | 6932 |
| clone | 1B4E9 |
| WB Predicted band size | 41.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TCF7 (AA: 168-358) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TCF7抗体的3篇参考文献的简要总结(内容基于公开研究归纳，非真实文献)：
1. **文献名称**: "TCF7 regulates immune cell differentiation through Wnt signaling"
**作者**: Smith et al.
**摘要**: 研究利用TCF7抗体通过流式细胞术和Western blot分析，揭示TCF7在T细胞分化中的关键作用，发现其缺失导致CD8+ T细胞功能受损，影响抗肿瘤免疫应答。
2. **文献名称**: "TCF7 expression in melanoma correlates with immune evasion"
**作者**: Wang et al.
**摘要**: 通过TCF7抗体的免疫组化染色，发现黑色素瘤中TCF7高表达与PD-1/PD-L1通路激活相关，提示TCF7可能作为免疫治疗耐药性的潜在生物标志物。
3. **文献名称**: "TCF7 antibody-based profiling in colorectal cancer stem cells"
**作者**: Lee et al.
**摘要**: 研究使用TCF7抗体富集结直肠癌干细胞，证实TCF7通过调控Wnt/β-catenin通路维持干细胞自我更新能力，靶向TCF7可抑制肿瘤生长。
(注：以上内容为示例，实际文献需通过PubMed/Google Scholar等平台检索确认。)
**Background of TCF7 Antibody**
TCF7 (T-cell factor 7), also known as TCF-1. is a transcription factor encoded by the *TCF7* gene and belongs to the TCF/LEF (lymphoid enhancer-binding factor) family. It plays a critical role in the Wnt/β-catenin signaling pathway, where it interacts with β-catenin to regulate the transcription of target genes involved in cell proliferation, differentiation, and survival. TCF7 is particularly essential in immune system development, notably in T-cell lineage commitment and thymocyte differentiation. It is also implicated in maintaining stem cell pluripotency and has dual roles in cancer, acting as either a tumor suppressor or promoter depending on the context.
TCF7 antibodies are tools designed to detect and study the expression, localization, and function of the TCF7 protein. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers utilize TCF7 antibodies to investigate its role in immune disorders, cancer biology (e.g., T-cell acute lymphoblastic leukemia, colorectal cancer), and stem cell regulation. Commercial TCF7 antibodies are often raised against specific epitopes, with host species (e.g., mouse, rabbit) and clonal variants (monoclonal or polyclonal) tailored for diverse experimental needs. Validation of antibody specificity is crucial due to potential cross-reactivity with other TCF/LEF family members. Studies using TCF7 antibodies have significantly advanced understanding of Wnt signaling dynamics and its therapeutic targeting.
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