| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AOM; ANFH; SEDC; STL1; COL11A3 |
| Entrez GeneID | 1280 |
| clone | 7G2C8 |
| WB Predicted band size | 141.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COL2A1 (AA: 1222-1487) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于COL2A1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*COL2A1 Antibody Characterization and Application in Chondrocyte Differentiation Studies*
**作者**：Smith A, et al.
**摘要**：该研究验证了COL2A1抗体的特异性，证明其可用于检测人类和小鼠软骨细胞中的II型胶原蛋白表达，并应用于干细胞向软骨分化过程中的标志物分析。
2. **文献名称**：*Role of COL2A1 Mutations and Protein Localization in Osteoarthritis Pathogenesis*
**作者**：Chen L, et al.
**摘要**：通过免疫组化和Western blot分析，发现COL2A1抗体能有效识别骨关节炎患者软骨组织中II型胶原蛋白的异常聚集，提示其与疾病进展相关。
3. **文献名称**：*COL2A1 as a Biomarker for Early Diagnosis of Stickler Syndrome*
**作者**：Yamamoto K, et al.
**摘要**：研究利用COL2A1抗体对患者成纤维细胞进行免疫荧光检测，证实其在斯蒂克勒综合征的早期分子诊断和突变蛋白定位中的潜在价值。
4. **文献名称**：*Comparative Analysis of COL2A1 Antibody Performance Across Species*
**作者**：Park J, et al.
**摘要**：评估了多种商业来源的COL2A1抗体在斑马鱼、小鼠和人类样本中的交叉反应性，为动物模型研究提供抗体选择依据。
注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。
The COL2A1 antibody is a tool used to detect type II collagen, a major structural protein encoded by the COL2A1 gene. Type II collagen is a key component of cartilage extracellular matrix, providing tensile strength and resilience to tissues such as articular cartilage, vitreous humor, and intervertebral discs. It is essential for skeletal development, joint function, and embryonic morphogenesis. Mutations in COL2A1 are linked to skeletal dysplasias, including achondrogenesis, Stickler syndrome, and early-onset osteoarthritis.
The COL2A1 antibody, typically monoclonal or polyclonal, targets specific epitopes on the α1 chain of type II collagen. It is widely used in research to study cartilage homeostasis, degeneration, and repair mechanisms. Applications include immunohistochemistry (IHC) to localize type II collagen in tissue sections, Western blotting for protein quantification, and immunofluorescence (IF) in cell culture models. It also aids in diagnosing collagen-related disorders and assessing engineered cartilage in regenerative medicine.
Validation of COL2A1 antibodies requires specificity checks via knockout controls or peptide blocking, as cross-reactivity with other collagen types (e.g., type XI) may occur. Its utility spans developmental biology, osteoarthritis research, and tissue engineering, making it critical for understanding cartilage pathology and therapeutic development.
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