| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | hN2; AGS2; HJCYS |
| Entrez GeneID | 4853 |
| clone | 4C5A8 |
| WB Predicted band size | 265.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NOTCH2 (AA:extra 1391-1677) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于 **NOTCH2抗体** 的模拟参考文献示例(仅供格式参考，非真实文献)：
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1. **文献名称**: *NOTCH2 Antibody Targeting Inhibits Hepatocellular Carcinoma Progression via Suppressing STAT3-Hes1 Signaling*
**作者**: Li Y, et al.
**摘要**: 本研究利用特异性抗NOTCH2抗体阻断肝癌细胞中NOTCH2信号通路，发现其通过抑制下游Hes1和STAT3磷酸化显著降低肿瘤增殖和转移，为肝癌靶向治疗提供新策略。
2. **文献名称**: *Structural Characterization of NOTCH2 Extracellular Domain and Antibody-Based Therapeutic Potential in Biliary Atresia*
**作者**: Gurney A, et al.
**摘要**: 通过X射线晶体学解析NOTCH2胞外结构域，开发出单克隆抗体以阻断配体结合，并在胆道闭锁小鼠模型中证实其可改善肝脏纤维化及胆管发育异常。
3. **文献名称**: *Dual Role of NOTCH2 Antibody in Tumor Angiogenesis and Chemoresistance in Pancreatic Cancer*
**作者**: Kangsamaksin T, et al.
**摘要**: 研究显示，NOTCH2抗体可抑制胰腺癌中异常血管生成，并通过下调VEGF表达增强化疗药物敏感性，提示其在联合治疗中的应用潜力。
4. **文献名称**: *NOTCH2 Antibody Reveals Developmental Defects in Alagille Syndrome Models*
**作者**: Hofmann JJ, et al.
**摘要**: 利用NOTCH2抗体染色技术，发现Alagille综合征模型中NOTCH2信号缺失导致胆管发育障碍，为疾病机制研究提供分子依据。
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**注**: 以上文献为模拟示例，实际引用时需通过PubMed/Google Scholar等平台检索真实文献。建议使用关键词“NOTCH2 antibody + 疾病/机制”进一步筛选。
The NOTCH2 antibody is a key tool for studying the NOTCH2 receptor, a critical component of the Notch signaling pathway. NOTCH2. a transmembrane protein, belongs to the evolutionarily conserved Notch receptor family, which regulates cell-cell communication, differentiation, proliferation, and apoptosis. It plays essential roles in embryonic development, tissue homeostasis, and stem cell regulation. Dysregulation of NOTCH2 is linked to developmental disorders (e.g., Alagille syndrome), cancers (e.g., lymphoma, breast cancer), and cardiovascular diseases.
NOTCH2 antibodies are designed to detect and analyze the expression, localization, and activation of NOTCH2 in various biological samples. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies help identify NOTCH2 cleavage events, receptor-ligand interactions, and downstream signaling activity. Specific monoclonal or polyclonal antibodies target distinct domains (e.g., extracellular, intracellular) to study structure-function relationships.
Researchers also use NOTCH2 antibodies to explore therapeutic targeting, as aberrant Notch signaling is implicated in tumor progression and resistance to therapy. However, challenges remain in ensuring antibody specificity and minimizing cross-reactivity with other Notch family members (NOTCH1. 3. 4). Validated antibodies are crucial for diagnostic applications, such as detecting NOTCH2 mutations or overexpression in clinical samples. Ongoing studies aim to refine antibody-based tools for precision medicine and drug development targeting the Notch pathway.
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