Mouse Monoclonal NOTCH2 Antibody

The NOTCH2 antibody is a crucial tool for studying the NOTCH2 receptor, a key component of the NOTCH signaling pathway involved in cell-cell communication, differentiation, and tissue development. NOTCH2. one of four mammalian NOTCH receptors (NOTCH1-4), regulates processes like embryogenesis, stem cell maintenance, and immune cell function. Dysregulation of NOTCH2 is linked to cancers (e.g., breast, lung, and gliomas) and developmental disorders like Alagille syndrome and Hajdu-Cheney syndrome. Antibodies targeting NOTCH2 enable researchers to detect its expression, localization, and activation status in tissues or cells using techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies typically recognize specific domains (e.g., extracellular, intracellular) or post-translational modifications (e.g., cleaved forms), helping to dissect NOTCH2’s role in health and disease. Validating antibody specificity is critical, as NOTCH receptors share structural homology, and cross-reactivity with other NOTCH family members (e.g., NOTCH1) may lead to misinterpretation. Many commercial NOTCH2 antibodies are monoclonal or polyclonal, with validation data including knockout cell lines or tissue controls. Recent studies also utilize NOTCH2 antibodies to explore therapeutic targeting, such as inhibitory antibodies in cancer or agonists for tissue repair. Researchers must consider species reactivity, epitope conservation, and experimental conditions when selecting antibodies for reproducible results.