| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | K14; NFJ; CK14; EBS1; EBS3; EBS4; EBS1A; EBS1B; EBS1C; EBS1D |
| Entrez GeneID | 3861 |
| clone | 4D2A5 |
| WB Predicted band size | 51.5kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT14 (AA: 115-472) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与KRT14抗体相关的代表性文献及摘要：
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1. **文献名称**: *"A mutation in human keratin K14 causes epidermolysis bullosa simplex"*
**作者**: Coulombe, P.A., Hutton, M.E., Fuchs, E.
**摘要**: 该研究首次报道了KRT14基因突变导致人类遗传性皮肤病——单纯型大疱性表皮松解症(EB simplex)。作者利用KRT14抗体证实突变蛋白在患者表皮基底细胞中表达异常，破坏角蛋白中间丝结构，导致皮肤机械脆弱性增加。
2. **文献名称**: *"Keratin 14 and 17 as diagnostic biomarkers in squamous cell carcinoma"*
**作者**: Chu, P.G., Weiss, L.M.
**摘要**: 文章通过免疫组化分析，验证了KRT14抗体在区分鳞状细胞癌(SCC)与其他上皮源性肿瘤(如腺癌)中的特异性。研究发现，KRT14在SCC中高表达，可作为辅助病理诊断的标志物。
3. **文献名称**: *"KRT14 marks a subpopulation of aggressive basal-like breast cancers"*
**作者**: Laakso, M., Tanner, M., Nilsson, J.
**摘要**: 该研究利用KRT14抗体识别基底样乳腺癌亚型，发现KRT14阳性肿瘤表现出更高的转移倾向和化疗耐药性，提示其可作为预后不良的分子标记及潜在治疗靶点。
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**扩展领域补充**：
4. **文献名称**: *"Catalog of 320 antibodies against keratins in normal and pathologic epithelia"*
**作者**: Moll, R., Franke, W.W., Schiller, D.L.
**摘要**: 该文献系统整理了包括KRT14在内的多种角蛋白抗体在不同上皮组织(正常/病变)中的表达谱，强调KRT14抗体在鉴定基底上皮细胞及相应肿瘤中的关键作用。
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这些文献涵盖了KRT14抗体在遗传病机制、癌症诊断分型及基础研究中的核心应用。如需具体文章DOI或发表年份，可进一步补充关键词进行精准检索。
Keratin 14 (KRT14) is a type I intermediate filament protein encoded by the *KRT14* gene, playing a critical role in maintaining structural integrity of epithelial cells. It pairs with keratin 5 (KRT5) to form a cytoskeletal network in basal keratinocytes, providing mechanical resilience to tissues subjected to stress, such as skin and mucosal epithelia. KRT14 mutations are linked to epidermolysis bullosa simplex (EBS), a blistering skin disorder caused by cytoskeletal fragility.
KRT14 antibodies are immunodetection tools targeting this protein, widely used in research and diagnostics. Monoclonal or polyclonal antibodies enable visualization of KRT14 expression via immunohistochemistry (IHC) or immunofluorescence (IF), aiding in identifying basal epithelial cells in tissues. These antibodies are essential in studying epithelial differentiation, wound healing, and cancer biology, as KRT14 is a marker of stratified squamous epithelia and certain carcinomas.
Clinically, KRT14 antibodies assist in diagnosing EBS and differentiating skin cancers (e.g., squamous vs. adenocarcinoma). They also contribute to understanding epithelial-mesenchymal transition (EMT) in metastasis. Validation of antibody specificity remains crucial due to keratin family homology. Overall, KRT14 antibodies serve as vital reagents in epithelial biology research and pathological diagnostics.
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