| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | APS |
| Entrez GeneID | 10603 |
| clone | 5E11G7 |
| WB Predicted band size | 67.7kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SH2B2 (AA: 497-676) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SH2B2抗体的3篇参考文献及其摘要内容：
1. **"SH2B2/APS regulates immune responses through binding to MHC class II molecules"**
**作者**: Li et al. (2015)
**摘要**: 该研究通过生成特异性SH2B2抗体，揭示了SH2B2在抗原呈递细胞中的表达及其与MHC II分子的相互作用，证明其通过调控T细胞活化影响免疫应答。
2. **"The role of SH2B2 in leptin signaling and obesity-related metabolic disorders"**
**作者**: Smith et al. (2018)
**摘要**: 利用SH2B2抗体进行免疫沉淀实验，研究发现SH2B2通过结合瘦素受体调控下游信号通路，影响能量代谢，并与肥胖相关代谢紊乱密切相关。
3. **"Development and characterization of monoclonal antibodies against SH2B2 for cancer immunotherapy"**
**作者**: Wang et al. (2020)
**摘要**: 研究开发了靶向SH2B2的单克隆抗体，并在肿瘤模型中验证其抗肿瘤活性，表明其可通过阻断SH2B2介导的信号通路抑制癌细胞增殖。
注：上述文献信息为模拟示例，实际引用时需核对真实来源及准确性。
SH2B2. also known as APS (Adaptor Protein with Pleckstrin Homology and Src Homology 2 Domains), is a member of the SH2B family of adaptor proteins involved in signal transduction pathways. It contains critical functional domains, including a pleckstrin homology (PH) domain, an SH2 domain, and a C-terminal dimerization domain, enabling interactions with tyrosine kinase receptors and downstream signaling components. SH2B2 is primarily expressed in metabolic tissues, such as adipose tissue, liver, and skeletal muscle, where it regulates signaling cascades initiated by insulin, leptin, and growth factors. Studies suggest it modulates receptor tyrosine kinase activity, influencing cell proliferation, differentiation, and metabolic homeostasis. Dysregulation of SH2B2 has been linked to metabolic disorders, including diabetes and obesity, as well as certain cancers.
Antibodies targeting SH2B2 are essential tools for investigating its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to study SH2B2’s role in cellular processes. For example, SH2B2 antibodies help elucidate mechanisms by which SH2B2 enhances or inhibits receptor tyrosine kinase signaling, such as its interaction with insulin receptors or Trk neurotrophin receptors. Additionally, these antibodies aid in exploring SH2B2’s involvement in immune regulation, as it interacts with T-cell receptor signaling components. The development of specific, high-affinity SH2B2 antibodies continues to support research into metabolic diseases, cancer biology, and therapeutic targeting of signaling pathways.
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