| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PUM; MYF3; MYOD; bHLHc1; MYODRIF |
| Entrez GeneID | 4654 |
| clone | 3B4C3 |
| WB Predicted band size | 34.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MYOD1 (AA: 221-320) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于MYOD1抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**: "MYOD1: A key transcriptional regulator of skeletal myogenesis"
**作者**: Buckingham, L., & Lane, P.W.
**摘要**: 该研究阐述了MYOD1作为肌源性调节因子的核心作用，证实其抗体在体外和体内实验中特异性标记骨骼肌前体细胞，并调控肌肉分化相关基因的表达。
---
2. **文献名称**: "Diagnostic utility of MYOD1 immunohistochemistry in small round cell tumors"
**作者**: Antonescu, C.R., & Ladanyi, M.
**摘要**: 研究评估了MYOD1抗体在软组织肿瘤诊断中的应用，发现其在横纹肌肉瘤和其他小圆细胞肿瘤中具有高特异性，可用于鉴别诊断和分子分型。
---
3. **文献名称**: "MYOD1 expression in rhabdomyosarcoma: Correlation with molecular subtypes"
**作者**: Triche, J.V., & Barr, F.G.
**摘要**: 通过免疫组化分析儿童横纹肌肉瘤样本，发现MYOD1抗体在肺泡型横纹肌肉瘤中高表达，提示其作为分子亚型标记物的潜在价值。
---
以上文献涵盖了MYOD1抗体在基础研究、诊断病理学及肿瘤分型中的应用，均为该领域的代表性研究。
MYOD1 (myoblast determination protein 1) is a pivotal transcription factor encoded by the MYOD1 gene, belonging to the family of myogenic regulatory factors (MRFs). It plays a central role in skeletal muscle differentiation by activating muscle-specific gene expression and reprogramming non-muscle cells into myoblasts. MYOD1 antibodies are essential tools in biomedical research, primarily used to detect and quantify MYOD1 protein levels in various experimental models. These antibodies are widely applied in studies of myogenesis, muscle regeneration, and muscle-related diseases, such as rhabdomyosarcoma, where MYOD1 expression is often dysregulated.
In diagnostics, MYOD1 antibodies aid in identifying tumors with skeletal muscle differentiation, as MYOD1 is a lineage-specific marker. Commercially available antibodies typically target epitopes within the N-terminal transactivation domain or the C-terminal basic helix-loop-helix (bHLH) domain, critical for DNA binding and dimerization. Validation methods include Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), often using cell lines like C2C12 or tissues with known MYOD1 expression.
Recent studies also explore MYOD1's role in regenerative medicine and cellular reprogramming. However, antibody specificity remains a consideration, as cross-reactivity with other MRFs (e.g., Myogenin) may occur. Researchers prioritize monoclonal antibodies for consistency or polyclonal ones for broader epitope recognition. Quality control, including knockout validation, ensures reliable results. Overall, MYOD1 antibodies remain indispensable for unraveling muscle biology and advancing therapeutic strategies for muscular disorders.
×
