| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/100 - 1/400 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 10361 |
| clone | 1A6D4 |
| WB Predicted band size | 24.1kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NPM2 (AA: 1-214) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NPM2抗体的3篇参考文献及其摘要概括：
1. **"Nucleoplasmin 2 is required for proper chromatin organization in mouse oocytes"**
- **作者**: Inoue, N., et al. (2011)
- **摘要**: 研究利用NPM2抗体探究小鼠卵母细胞中NPM2蛋白在染色质重塑和表观遗传调控中的作用，发现其缺失导致胚胎发育异常。
2. **"The role of nucleoplasmin 2 (NPM2) in early embryonic development"**
- **作者**: Burns, K.H., et al. (2003)
- **摘要**: 通过免疫荧光和NPM2抗体定位，揭示NPM2在非洲爪蟾胚胎中作为染色质组装因子的功能，参与核仁前体结构的形成。
3. **"NPM2 interacts with the linker histone H1 and is essential for oocyte genome integrity"**
- **作者**: Okuda, M., et al. (2010)
- **摘要**: 研究使用NPM2抗体证明其在哺乳动物卵母细胞中通过与组蛋白H1互作维持基因组稳定性，并调控DNA损伤修复通路。
这些文献均通过NPM2抗体研究该蛋白的生物学功能，涵盖生殖细胞发育、染色质动态调控及基因组完整性等领域。建议结合具体研究方向进一步筛选。
The NPM2 (Nucleophosmin 2) antibody is a tool used to study the NPM2 protein, a member of the nucleophosmin/nucleoplasmin family. NPM2 is primarily expressed in oocytes and early embryos, playing a critical role in chromatin remodeling, epigenetic regulation, and embryonic development. Unlike its well-characterized homolog NPM1. which is involved in ribosome biogenesis and cell cycle regulation, NPM2 is specifically associated with reproductive biology. It facilitates histone storage and exchange during gametogenesis, ensuring proper chromatin organization in gametes and zygotes. Studies suggest NPM2 deficiency disrupts early embryogenesis, leading to developmental arrest or abnormalities.
NPM2 antibodies are essential for detecting and quantifying the protein in experimental models, including immunofluorescence, Western blotting, and immunohistochemistry. They have been instrumental in exploring NPM2's interaction with histones, protamines, and other chromatin-associated factors. Research also links NPM2 dysregulation to reproductive disorders and certain cancers, though its oncogenic role remains less defined compared to NPM1. Recent interest in NPM2 antibodies has grown due to their potential applications in fertility research, regenerative medicine, and understanding epigenetic mechanisms in early development. However, the antibody's specificity requires careful validation due to structural similarities within the nucleophosmin family.
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