| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 58157 |
| clone | 8C2H4 |
| WB Predicted band size | 16.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human NGB(AA: full 1-151) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于Neuroglobin(NGB)抗体的关键参考文献，内容涵盖其应用及功能研究：
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1. **文献名称**：*Neuroglobin and cytoglobin: Genes, proteins and evolution*
**作者**：Burmester T, et al.
**摘要**：该研究首次报道了神经球蛋白(NGB)的发现，并开发了特异性抗体用于检测其在中枢神经系统中的分布。通过免疫组化证实NGB在神经元中高表达，提示其在缺氧条件下的潜在保护作用。
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2. **文献名称**：*Neuroglobin protects the brain from experimental stroke in vivo*
**作者**：Sun Y, et al.
**摘要**：利用NGB特异性抗体进行Western blot和免疫荧光分析，发现脑缺血小鼠模型中NGB表达上调。研究证明NGB通过减少氧化应激和凋亡发挥神经保护功能。
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3. **文献名称**：*Neuroglobin: A novel player in the oxidative stress response of cancer cells*
**作者**：Watanabe S, et al.
**摘要**：通过抗体验证NGB在胶质瘤细胞中的亚细胞定位(线粒体和细胞质)，并发现其通过调节活性氧(ROS)水平抑制肿瘤进展，为NGB的抗氧化机制提供了新证据。
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**提示**：如需获取全文，可通过PubMed或Web of Science搜索标题/作者。早期研究(如Burmester, 2003)多聚焦抗体开发和基础分布，后续研究(如Sun, 2010)则深入探索其功能机制。
Neuroglobin (NGB), a member of the globin family, is a small heme-binding protein predominantly expressed in vertebrate nervous systems. Discovered in 2000. NGB shares structural similarities with hemoglobin and myoglobin but exhibits distinct oxygen-binding properties and tissue-specific roles. It is implicated in neuroprotection under hypoxic or oxidative stress conditions, potentially through mechanisms like oxygen storage/scavenging, reactive oxygen species (ROS) neutralization, and modulation of mitochondrial function.
NGB antibodies have become essential tools for exploring its expression patterns, cellular localization, and pathophysiological functions. Studies using these antibodies revealed elevated NGB levels in brain regions vulnerable to ischemic injury, suggesting its adaptive response to hypoxia. In disease models (e.g., Alzheimer's, Parkinson's, stroke), NGB antibodies help quantify protein fluctuations, correlating with neuronal survival or damage.
Commercial and custom-generated NGB antibodies enable techniques like Western blot, immunohistochemistry, and ELISA. Species cross-reactivity (e.g., human, rodent, avian) observed in many antibodies highlights NGB's evolutionary conservation. Recent research focuses on NGB's interactions with signaling molecules and its potential as a therapeutic biomarker. Despite unresolved questions about its precise mechanisms, NGB antibodies remain crucial for unraveling its role in neural homeostasis and pathology.
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