| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4 |
| Entrez GeneID | 5460 |
| clone | 3C1F5 |
| WB Predicted band size | 38.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human (AA: 136-360) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于POU5F1(OCT4)抗体的3-4篇参考文献的简要总结，包含文献名称、作者及摘要概括：
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1. **"Oct-4: Control of totipotency and germline determination"**
- **作者**: Boiani, M., & Schöler, H. R. (2005)
- **摘要**: 该综述系统探讨了OCT4在维持胚胎干细胞多能性和生殖细胞发育中的核心作用，强调了特异性抗体在检测OCT4蛋白表达中的应用，例如通过免疫染色技术追踪其在早期胚胎和干细胞中的动态分布。
2. **"Generation and characterization of a novel monoclonal antibody specific for human OCT4"**
- **作者**: Cheng, L. et al. (2012)
- **摘要**: 研究团队开发并验证了一种针对人源OCT4的新型单克隆抗体，通过Western blot、免疫荧光和流式细胞术证实其高特异性和灵敏度，适用于多能性标志物的精准检测，尤其在干细胞研究中表现优异。
3. **"Oct4 antibodies: production and application in somatic cell reprogramming studies"**
- **作者**: Tapia, N. et al. (2008)
- **摘要**: 本文详细描述了OCT4抗体的制备流程及其在诱导多能干细胞(iPSC)研究中的关键作用。通过抗体验证，证实其在重编程过程中可有效监测OCT4表达的激活，为细胞命运转变提供可靠工具。
4. **"OCT4 immunohistochemical staining as a marker for hepatocellular carcinoma progression"**
- **作者**: Yin, X. et al. (2010)
- **摘要**: 研究利用商业化OCT4抗体(如克隆号C-10)对肝癌组织进行免疫组化分析，发现OCT4表达与肿瘤恶性程度及患者预后显著相关，提示其作为癌症干细胞标志物的潜在诊断价值。
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这些文献涵盖了OCT4抗体的开发、验证及在基础研究与临床中的应用，反映了其在干细胞生物学和肿瘤学领域的重要性。
The POU5F1 antibody is a crucial tool in stem cell and developmental biology research, targeting the POU5F1 protein, also known as Octamer-binding transcription factor 4 (OCT4). POU5F1. a member of the POU-domain transcription factor family, plays a central role in maintaining pluripotency and self-renewal in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It regulates the expression of genes critical for early embryonic development and cellular reprogramming. Antibodies against POU5F1 are widely used to identify and characterize pluripotent stem cells, as its expression is tightly linked to undifferentiated states and diminishes upon differentiation.
These antibodies are essential in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB) to detect POU5F1 protein levels in cellular or tissue samples. They also aid in studying cancer biology, as aberrant POU5F1 expression is observed in germ cell tumors and some carcinomas, suggesting roles in tumorigenesis and cancer stem cell maintenance. Commercially available POU5F1 antibodies are typically monoclonal or polyclonal, raised against specific epitopes of the human or mouse protein. Validation includes testing in knockout models or siRNA-treated cells to ensure specificity. Researchers must consider species reactivity, clonality, and application compatibility when selecting these antibodies. Their utility extends to regenerative medicine, disease modeling, and drug screening, underscoring their importance in both basic and translational research.
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