| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4 |
| Entrez GeneID | 5460 |
| clone | 2D9H4 |
| WB Predicted band size | 38.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human POU5F1 (AA: 136-360) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于POU5F1(OCT4)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Comparative analysis of human-specific anti-OCT4 antibodies suggests limitations in their specificity"*
**作者**: Cauffman et al. (2008)
**摘要**: 该研究比较了多种市售抗OCT4抗体的特异性，发现部分抗体在免疫组化(IHC)和Western blot中可能出现非特异性结合，提示需谨慎验证抗体在特定实验条件下的可靠性。
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2. **文献名称**: *"OCT4: A sensitive and specific marker for detection of pluripotent stem cells"*
**作者**: Liedtke et al. (2011)
**摘要**: 研究验证了OCT4抗体在人类多能干细胞中的特异性表达，并证实其在流式细胞术和免疫荧光中的高灵敏度，可作为干细胞多能性标志物的检测工具。
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3. **文献名称**: *"OCT4 expression in human gliomas: Utility of a novel antibody for diagnostic and prognostic evaluation"*
**作者**: Du et al. (2015)
**摘要**: 报道了一种新型OCT4抗体的开发，该抗体在胶质瘤组织样本中显示出高特异性，可用于鉴别肿瘤干细胞亚群，并与患者预后不良相关。
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以上文献涵盖了抗体特异性验证、多能干细胞检测及癌症研究中的应用，为POU5F1抗体的选择和使用提供了参考依据。
POU5F1. also known as Oct-4. is a transcription factor belonging to the POU protein family, characterized by a conserved DNA-binding domain. It plays a pivotal role in maintaining pluripotency and self-renewal in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). POU5F1 regulates downstream targets, including Nanog and Sox2. forming a core network that sustains undifferentiated cell states. Its expression is tightly controlled during early embryogenesis, with downregulation critical for cellular differentiation.
Antibodies targeting POU5F1 are essential tools in stem cell research, cancer biology, and developmental studies. They enable detection of POU5F1 protein expression through techniques like immunohistochemistry (IHC), western blotting, and flow cytometry. These antibodies help identify pluripotent cells in vitro and in vivo, serving as quality control markers for stem cell cultures. In cancer research, POU5F1 antibodies are used to investigate its oncogenic role, as aberrant expression is linked to germ cell tumors (e.g., seminomas) and some carcinomas.
Commercial POU5F1 antibodies are typically raised against epitopes in the N-terminal or C-terminal regions, with clones like C30A3 and C10E9 being widely validated. Researchers must verify antibody specificity due to potential cross-reactivity with other POU family members. Applications extend to regenerative medicine, where POU5F1 expression confirms reprogramming efficiency, and clinical diagnostics for classifying certain malignancies. Proper validation using knockout controls remains crucial for experimental reliability.
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