| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AOM; ANFH; SEDC; STL1; COL11A3 |
| Entrez GeneID | 1280 |
| clone | 7F2A11 |
| WB Predicted band size | 142kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COL2A1 (AA: 1222-1487) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于COL2A1抗体的3篇参考文献摘要，按研究领域分类整理：
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### 1. **发育与疾病模型**
**文献名称**：*COL2A1 Antibodies in Chondrodysplasia: Diagnostic Utility in Mouse Models*
**作者**：Smith et al.
**摘要**：研究利用COL2A1特异性抗体分析Ⅱ型胶原在转基因小鼠软骨发育异常中的表达变化，证实抗体在骨骼畸形诊断中的有效性，为遗传性软骨发育不良提供分子检测依据。
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### 2. **眼科研究**
**文献名称**：*Immunolocalization of Type II Collagen in Vitreous Disorders Using COL2A1-Specific Antibodies*
**作者**：Chen & Johnson
**摘要**：通过COL2A1抗体免疫组化技术，揭示玻璃体病变中Ⅱ型胶原的异常分布，提示其与视网膜脱离等疾病的关联，为眼科病理机制研究提供新视角。
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### 3. **癌症生物学**
**文献名称**：*COL2A1 as a Biomarker in Chondrosarcoma: Antibody-Based Detection and Clinical Correlations*
**作者**：Wang et al.
**摘要**：研究验证COL2A1抗体在软骨肉瘤组织中的高特异性，发现Ⅱ型胶原表达水平与肿瘤分化程度显著相关，提出其作为预后评估的潜在标志物。
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**注**：以上为示例性内容，实际文献需通过PubMed或Web of Science检索关键词“COL2A1 antibody”+具体应用场景获取。如需全文协助，可提供更具体的研究方向进一步筛选。
The COL2A1 antibody is a crucial tool for studying type II collagen, a major structural protein encoded by the COL2A1 gene. Type II collagen is predominantly found in hyaline cartilage, vitreous humor of the eye, and intervertebral discs, playing a vital role in maintaining tissue integrity and elasticity. It is essential for skeletal development, joint function, and embryonic growth. Mutations in COL2A1 are linked to genetic disorders such as Stickler syndrome, achondrogenesis, and spondyloepiphyseal dysplasia, characterized by skeletal abnormalities, vision issues, or hearing loss.
Researchers use COL2A1 antibodies to detect and localize type II collagen in tissues, aiding in the diagnosis and study of cartilage-related pathologies like osteoarthritis, degenerative disc disease, and cartilage repair mechanisms. These antibodies are widely applied in techniques such as immunohistochemistry, Western blotting, and immunofluorescence. Specificity validation is critical, as cross-reactivity with other collagen types (e.g., types XI or IX) may occur due to structural similarities. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity in certain assays. Recent advances in cartilage regeneration and bioengineering, such as stem cell therapy or 3D bioprinting, also rely on COL2A1 antibodies to assess extracellular matrix production and tissue maturation. Proper antibody selection and validation ensure accurate insights into cartilage biology and disease mechanisms.
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