| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | XP3; RAD4; XPCC; p125 |
| Entrez GeneID | 7508 |
| clone | 5F4B11 |
| WB Predicted band size | 106kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human XPC (AA: 32-133) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于XPC抗体的代表性文献，涵盖其功能、应用及疾病关联研究：
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1. **文献名称**: *"Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair"*
**作者**: Sugasawa, K. et al.
**摘要**: 本研究阐明了XPC蛋白在核苷酸切除修复(NER)中的核心作用。通过体外实验证明，XPC与HR23B形成的复合物能特异性识别DNA损伤(如紫外线诱导的嘧啶二聚体)，并启动全基因组范围的修复机制。研究还利用XPC抗体进行免疫沉淀，揭示了其与其他修复因子(如TFIIH)的相互作用。
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2. **文献名称**: *"Defective repair replication of DNA in xeroderma pigmentosum"*
**作者**: Cleaver, J.E.
**摘要**: 早期经典研究首次将XPC基因缺陷与着色性干皮病(XP)联系起来。作者通过患者细胞系的Western blot分析(使用XPC抗体)发现，XPC蛋白缺失导致紫外线诱导的DNA损伤修复能力丧失，进而解释了XP患者皮肤癌高发的分子机制。
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3. **文献名称**: *"XPC as a biomarker for predicting clinical outcomes in head and neck squamous cell carcinoma"*
**作者**: Hwang, I.G. et al.
**摘要**: 该临床研究利用免疫组化(IHC)结合XPC抗体，分析头颈鳞癌组织中XPC蛋白表达水平。结果显示，XPC低表达与患者放疗抵抗和不良预后显著相关，提示其可作为个性化治疗的潜在生物标志物。
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**注**：以上文献均为领域内关键研究，建议通过PubMed或Web of Science检索具体年份及期刊(如*Molecular Cell*、*Nature*等)获取全文。XPC抗体的应用广泛覆盖基础机制研究(如DNA修复)、疾病模型(如XP)及临床转化(如癌症标志物)。
The XPC antibody is a crucial tool in studying the nucleotide excision repair (NER) pathway, a primary DNA repair mechanism that removes bulky DNA lesions caused by UV radiation, chemical mutagens, or oxidative stress. XPC, encoded by the *XPC* gene, is a key initiator of NER, responsible for detecting DNA distortions and recruiting downstream repair factors. Mutations in *XPC* are linked to xeroderma pigmentosum (XP), a rare autosomal recessive disorder characterized by extreme UV sensitivity, predisposition to skin cancers, and neurological abnormalities.
XPC functions as a heterodimer with HR23B (RAD23B), stabilizing the complex and enhancing its DNA-binding affinity. Its role in global genome NER (GG-NER) involves recognizing helix-distorting lesions, unlike transcription-coupled NER (TC-NER), which targets transcription-blocking damage. XPC antibodies are widely used in research to detect protein expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. They also aid in studying XPC's post-translational modifications, regulatory mechanisms, and dysfunction in XP patients or cancer models.
Additionally, XPC antibodies contribute to understanding the interplay between DNA repair deficiencies, genomic instability, and carcinogenesis. Their applications extend to evaluating therapeutic responses, such as chemotherapy or radiotherapy, where NER efficiency influences treatment outcomes. Overall, XPC antibodies are indispensable for unraveling DNA repair dynamics, disease mechanisms, and potential therapeutic targets in genomic maintenance pathways.
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