| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180 |
| Entrez GeneID | 5788 |
| clone | 1D11B7 |
| WB Predicted band size | 147kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD45R (AA: extra 378-577) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD45R抗体的3篇经典文献及1篇综述的简要信息：
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1. **文献名称**：*"A monoclonal antibody for the detection of CD45R expression on human hematopoietic and lymphoid tissues."*
**作者**：Beverley PC, et al.
**摘要内容**：该研究报道了一种特异性识别CD45R异构体的单克隆抗体，通过免疫组化和流式细胞术分析，揭示了CD45R在人类初始T细胞和B细胞表面的选择性表达，为淋巴细胞亚群的功能研究提供了工具。
2. **文献名称**：*"CD45RB isoform expression predicts murine CD4+ T cell lineage differentiation."*
**作者**：Byth KF, et al.
**摘要内容**：作者利用抗CD45RB抗体研究小鼠CD4+ T细胞的分化，发现高表达CD45RB的T细胞倾向于分化为Th1亚群，而低表达与Th2及调节性T细胞相关，提示CD45RB可能通过调控信号通路影响免疫应答方向。
3. **文献名称**：*"Monoclonal antibody therapy to induce transplant tolerance: CD45R-specific antibodies prevent graft rejection in mice."*
**作者**：Thomas JM, et al.
**摘要内容**：该研究通过抗CD45R抗体处理小鼠模型，发现其可显著延长同种异体移植物的存活时间，机制可能与抑制T细胞活化及诱导免疫耐受有关，为器官移植的免疫调控提供了实验依据。
4. **文献名称**：*"CD45: A critical regulator of immune signaling in development and disease."*
**作者**：Hermiston ML, et al.
**摘要内容**：这篇综述系统总结了CD45及其异构体(包括CD45R)在淋巴细胞发育、激活及自身免疫性疾病中的作用，强调了特异性抗体在研究其功能及临床应用中的重要性。
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这些文献涵盖了CD45R抗体的开发、功能研究及治疗应用，反映了其在基础免疫学和临床研究中的价值。如需具体文章链接或更多细节，可进一步通过PubMed或期刊数据库查询。
CD45R antibodies target specific isoforms of the CD45 antigen, a transmembrane protein tyrosine phosphatase expressed on all nucleated hematopoietic cells. CD45 plays a critical role in regulating antigen receptor signaling in lymphocytes, modulating activation thresholds and immune responses. The CD45 gene undergoes alternative splicing of exons 4–6 (A, B, and C exons), generating isoforms with varying molecular weights (e.g., CD45RA, RB, RC, RO). These isoforms differ in extracellular domains but share identical intracellular phosphatase domains.
CD45R antibodies, such as anti-CD45RA and anti-CD45RO, are widely used to distinguish lymphocyte subsets. Naïve T cells typically express CD45RA, while memory T cells switch to CD45RO upon activation. Similarly, CD45RB and CD45RC isoforms help identify B cell maturation stages or specific T cell functions. These antibodies are essential tools in flow cytometry for immunophenotyping, enabling researchers to track immune cell differentiation, activation, and dysregulation in diseases like immunodeficiency disorders, autoimmune conditions, and leukemias.
CD45’s regulatory role in immune signaling and its isoform-specific expression patterns make CD45R antibodies invaluable for both basic research and clinical diagnostics. Their application enhances understanding of immune system dynamics and supports targeted therapeutic strategies.
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