| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LAMPA; CD107a; LGP120 |
| Entrez GeneID | 3916 |
| clone | 4D2A6 |
| WB Predicted band size | 44.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human LAMP1 (AA: 182-414) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及LAMP1抗体的文献摘要概括：
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1. **文献名称**：*LAMP-1 and LAMP-2: Their role in autophagy and lysosomal biogenesis*
**作者**：Eskelinen EL et al.
**摘要**：该研究利用LAMP1抗体通过免疫荧光和免疫电镜技术，证实LAMP1是溶酶体膜的关键标记蛋白，并揭示其与自噬体-溶酶体融合过程的关联，为溶酶体功能研究提供方法学基础。
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2. **文献名称**：*Lysosomal membrane proteins and their central role in physiology*
**作者**：Saftig P, Klumperman J
**摘要**：通过LAMP1抗体的Western blot和共聚焦显微镜分析，作者发现LAMP1在维持溶酶体结构稳定性和细胞应激响应中的关键作用，并揭示其缺陷与神经退行性疾病的潜在联系。
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3. **文献名称**：*TFEB regulates lysosomal positioning by modulating LAMP1-mediated microtubule anchoring*
**作者**：Settembre C et al.
**摘要**：研究使用LAMP1抗体进行免疫沉淀和活细胞成像，证明LAMP1通过与微管结合蛋白相互作用调控溶酶体在细胞内的动态分布，进一步解析了溶酶体运动性的分子机制。
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4. **文献名称**：*LAMP1 controls SARS-CoV-2 entry via regulation of viral receptor ACE2 degradation*
**作者**：Chen J et al.
**摘要**：通过LAMP1抗体的流式细胞术和免疫组化分析，该文献提出LAMP1通过介导ACE2受体的溶酶体降解影响病毒入侵效率，为抗病毒治疗提供新靶点。
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以上文献均以LAMP1抗体为工具，涵盖基础机制(溶酶体动态、自噬)和疾病研究(病毒感染)，反映了其在多领域的应用价值。
**Background of LAMP1 Antibody**
Lysosome-associated membrane protein 1 (LAMP1) is a type I transmembrane glycoprotein predominantly localized in lysosomal membranes. It plays a critical role in maintaining lysosomal integrity, regulating autophagy, and mediating lysosome-phagosome fusion. Structurally, LAMP1 consists of two luminal domains heavily glycosylated to protect the lysosomal membrane from enzymatic degradation and a short cytoplasmic tail involved in intracellular trafficking.
LAMP1 antibodies are widely used as lysosomal markers in immunofluorescence, Western blotting, and flow cytometry to study lysosomal distribution, biogenesis, and dysfunction. These antibodies specifically target conserved epitopes in the luminal domain of LAMP1. enabling reliable detection across species like humans, mice, and rats.
Research applications include investigating lysosomal storage disorders, neurodegenerative diseases (e.g., Alzheimer’s), cancer (lysosomal exocytosis in metastasis), and immune responses (antigen presentation). Dysregulation of LAMP1 expression or trafficking is linked to pathological conditions, making it a biomarker for lysosomal activity and cellular stress.
Commercial LAMP1 antibodies vary in clonal specificity (e.g., H4A3. D2D11) and host species (mouse, rabbit), requiring validation for experimental contexts. Proper controls (e.g., knockout cells) are essential to confirm antibody specificity. Overall, LAMP1 antibodies are indispensable tools for exploring lysosome-related cellular mechanisms and disease pathogenesis.
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