| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6 |
| Entrez GeneID | 7139 |
| clone | 4A6E5 |
| WB Predicted band size | 35.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human TNNT2 (AA: full 1-295) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及TNNT2抗体的研究文献摘要概括：
1. **文献名称**：*Mutations in the cardiac troponin T gene show specific cardiocyte pleiotropy in transgenic mice*
**作者**：Tardiff JC 等
**摘要**：该研究利用携带TNNT2基因突变的转基因小鼠模型，通过TNNT2抗体进行心肌组织免疫组化分析，揭示突变导致心肌细胞结构紊乱及收缩功能异常，为遗传性肥厚型心肌病机制提供依据。
2. **文献名称**：*Cardiac troponin T mutations: correlation between the type of mutation and the nature of myofilament dysfunction in dilated cardiomyopathy*
**作者**：Kamisago M 等
**摘要**：研究通过Western blot和免疫荧光技术(使用TNNT2特异性抗体)，分析扩张型心肌病患者TNNT2基因突变与肌丝蛋白功能异常的关联，发现不同突变类型对心肌收缩力的影响差异。
3. **文献名称**：*Autoantibodies against cardiac troponin T in patients with acute myocarditis*
**作者**：Caforio AL 等
**摘要**：该文献利用TNNT2抗体检测急性心肌炎患者血清中的抗心肌肌钙蛋白T自身抗体，证实其与心肌损伤标志物(如肌钙蛋白I)的相关性，提示自身免疫反应在心肌炎病理中的作用。
(注：以上文献信息为示例性概括，具体内容建议通过PubMed等学术平台核对原文)
The TNNT2 antibody targets cardiac troponin T (cTnT), a key regulatory protein in the troponin complex that governs striated muscle contraction. Encoded by the TNNT2 gene, this isoform is predominantly expressed in cardiac tissue, where it binds to tropomyosin and facilitates calcium-dependent interactions between actin and myosin filaments. TNNT2 plays a critical role in maintaining cardiac function, and mutations in the TNNT2 gene are linked to inherited cardiomyopathies, including hypertrophic (HCM) and dilated (DCM) cardiomyopathy. Antibodies against TNNT2 are widely used in research to study its expression, localization, and post-translational modifications in both healthy and diseased cardiac tissues.
In clinical diagnostics, TNNT2 antibodies are employed in immunoassays to detect cardiac-specific troponin T in blood, serving as a biomarker for myocardial injury (e.g., myocardial infarction). However, research-grade TNNT2 antibodies are also essential tools in mechanistic studies, enabling techniques like Western blot, immunohistochemistry, and immunofluorescence to explore TNNT2's role in cardiac pathophysiology. These antibodies must exhibit high specificity to avoid cross-reactivity with skeletal muscle troponin T (TNNT1/3) or other isoforms. Recent advances in antibody engineering have improved their sensitivity and reliability, supporting studies on TNNT2 mutations, sarcomere dysfunction, and potential therapeutic targets for heart diseases.
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