| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Glutamate receptor ionotropic, NMDA 2C, GluN2C, Glutamate [NMDA] receptor subunit epsilon-3, N-methyl D-aspartate receptor subtype 2C, NMDAR2C, NR2C, GRIN2C, NMDAR2C |
| Entrez GeneID | 2905 |
| WB Predicted band size | 134.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This GRIN2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 567-595 amino acids from the Central region of human GRIN2C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NDC80抗体的3篇代表性文献的简要信息：
1. **文献名称**：*The conserved KMN network constitutes the core microtubule-binding site of the kinetochore*
**作者**：Cheeseman IM, et al.
**摘要**：研究阐明了NDC80复合体在有丝分裂动粒中的核心作用，利用特异性抗体(如抗NDC80抗体)验证其与微管的结合功能，并揭示了其在染色体分离中的关键机制。
2. **文献名称**：*NDC80 phosphorylation by Aurora kinase-A drives chromosomal instability in cancer*
**作者**：Deluca JG, et al.
**摘要**：通过抗NDC80抗体的免疫荧光和Western blot分析，证明Aurora激酶对NDC80的磷酸化调控异常可导致染色体不稳定，与肿瘤进展相关。
3. **文献名称**：*Development and validation of antibodies for the analysis of kinetochore proteins in human cells*
**作者**：McClelland SE, et al.
**摘要**：报道了针对NDC80等动粒蛋白的多种抗体的开发与验证，评估了抗体在免疫沉淀和活细胞成像中的特异性，为相关研究提供了工具支持。
这些文献通过NDC80抗体的应用，揭示了其在基础细胞生物学和疾病研究中的重要性。
The NDC80 antibody is a crucial tool for studying the NDC80 complex, a conserved heterotetrameric protein assembly essential for proper chromosome segregation during mitosis. Composed of NDC80 (also called HEC1), NUF2. SPC24. and SPC25 subunits, this complex forms part of the kinetochore structure, mediating stable attachments between spindle microtubules and chromosomes. Antibodies targeting NDC80 are widely used to investigate its role in cell cycle regulation, mitotic checkpoint control, and errors leading to aneuploidy.
These antibodies enable detection of NDC80 protein expression, localization, and interaction partners through techniques like Western blotting, immunofluorescence, and immunoprecipitation. In research, NDC80 antibodies have helped uncover its overexpression in various cancers, linking it to tumor progression and poor prognosis. They also facilitate studies on how phosphorylation regulates its microtubule-binding activity during different mitotic phases.
Commercial NDC80 antibodies are typically raised against specific epitopes in human NDC80. with cross-reactivity validated in common model organisms. Validation includes siRNA knockdown controls and functional assays. Challenges include distinguishing between phosphorylated isoforms and minimizing cross-reactivity with structurally related kinetochore proteins. As cancer therapies targeting mitotic machinery advance, NDC80 antibodies remain vital for probing mechanisms of chromosomal instability and evaluating potential therapeutic targets.
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