| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 |
| Entrez GeneID | 10403 |
| clone | 1D11B8 |
| WB Predicted band size | 74KDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in mammalian cells. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于NDC80抗体的3篇参考文献，简明列举如下：
1. **文献名称**：*The Conserved KMN Network Constitutes the Core Microtubule-Binding Site of the Kinetochore*
**作者**：Cheeseman, I.M. et al.
**摘要**：该研究解析了NDC80复合体在有丝分裂中与微管结合的关键功能，通过特异性抗体抑制实验，验证了其作为动粒核心组分的必要性，并揭示了复合体的结构-功能关系。
2. **文献名称**：*NDC80 Expression Is Associated with Poor Prognosis in Colorectal Cancer Patients*
**作者**：Dai, Y. et al.
**摘要**：利用NDC80抗体进行免疫组化分析，发现NDC80在结直肠癌组织中高表达，且与肿瘤分期和患者生存率显著相关，提示其作为潜在预后标志物的价值。
3. **文献名称**：*Phosphorylation Regulates the Binding of the NDC80 Complex to Kinetochores*
**作者**：DeLuca, J.G. et al.
**摘要**：通过磷酸化特异性抗体研究发现，NDC80复合体的磷酸化修饰调控其与动粒的结合能力，影响染色体分离的准确性，抗体干预实验进一步证实了其功能依赖性。
以上研究均通过NDC80抗体揭示了该蛋白在细胞分裂机制及疾病中的关键作用。
The NDC80 antibody targets a critical component of the NDC80 complex, a conserved, heterotetrameric protein assembly essential for proper chromosome segregation during mitosis. The complex, composed of NDC80 (also called HEC1), NUF2. SPC24. and SPC25. forms a key structural and functional unit of the kinetochore, mediating stable attachment of spindle microtubules to chromosomes. NDC80 itself plays a central role in establishing kinetochore-microtubule interactions, ensuring accurate chromosome alignment and segregation. Dysregulation of NDC80 function is linked to mitotic errors, genomic instability, and cancer progression, making it a focus of cell cycle and cancer biology research.
NDC80 antibodies are widely used to study kinetochore dynamics, mitotic checkpoint signaling, and mechanisms underlying aneuploidy. These antibodies enable detection of NDC80 localization via immunofluorescence, quantification of protein expression by Western blot, and exploration of protein-protein interactions through immunoprecipitation. Commercial NDC80 antibodies are typically raised against specific epitopes, such as the N-terminal region of human NDC80. and validated for species cross-reactivity (e.g., human, mouse, rat). Their applications extend to investigating therapeutic targets for cancer, as overexpression of NDC80 in tumors correlates with poor prognosis. Researchers often verify antibody specificity using knockout cell lines or siRNA-mediated knockdown to confirm signal loss, ensuring reliable experimental outcomes in studies of mitosis and genome integrity.
×
