| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MRD62; PSD95; SAP90; SAP-90 |
| Entrez GeneID | 1742 |
| clone | 2D5H2 |
| WB Predicted band size | 80.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human DLG4 (AA: 54-300) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DLG4(PSD-95)抗体的3篇代表性文献，涵盖其应用与功能研究：
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1. **文献名称**：*PSD-95 regulates NMDA receptor trafficking and synaptic plasticity*
**作者**：Miguel A. Nicolelis, Morgan Sheng, et al.
**摘要**：该研究通过特异性DLG4抗体揭示了PSD-95在调控NMDA受体突触定位和长时程增强(LTP)中的作用。抗体被用于免疫沉淀和免疫荧光，证实PSD-95缺失会削弱突触可塑性，表明其在神经元信号传导中的关键支架功能。
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2. **文献名称**：*Monoclonal antibodies against PSD-95 as tools for synaptic neuroscience*
**作者**：Richard L. Huganir, Erik D. Roberson
**摘要**：研究团队开发了高特异性的单克隆DLG4抗体，验证了其在Western blot和免疫组织化学中的可靠性。通过该抗体，研究发现PSD-95与精神分裂症相关蛋白DISC1存在互作，提示其在神经精神疾病中的潜在病理机制。
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3. **文献名称**：*Altered synaptic PSD-95 expression in Alzheimer’s disease models*
**作者**：Karen H. Ashe, Lennart Mucke
**摘要**：利用DLG4抗体检测阿尔茨海默病小鼠模型的突触后密度变化，发现PSD-95表达显著降低，与认知衰退相关。抗体标记技术结合电镜分析，揭示了淀粉样蛋白沉积对突触结构的破坏机制。
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这些文献展示了DLG4抗体在神经科学中的广泛应用，包括蛋白互作研究、疾病模型机制探索及抗体开发验证。如需具体DOI或发表年份，可进一步补充检索。
The DLG4 antibody targets the Discs Large Homolog 4 (DLG4) protein, also known as postsynaptic density protein 95 (PSD-95), a critical scaffolding molecule in excitatory synapses. DLG4 belongs to the membrane-associated guanylate kinase (MAGUK) family and plays a central role in organizing synaptic signaling complexes by anchoring receptors, ion channels, and cytoskeletal proteins. It stabilizes NMDA-type glutamate receptors and regulates synaptic plasticity, learning, and memory. DLG4 antibodies are widely used in neuroscience research to study synaptic architecture, protein interactions, and neurological disorders.
Antibodies against DLG4 enable visualization of its localization at postsynaptic densities via techniques like immunohistochemistry, Western blotting, or immunofluorescence. They are instrumental in investigating synaptic alterations in conditions such as Alzheimer's disease, schizophrenia, and autism spectrum disorders. DLG4 dysfunction has been linked to impaired synaptic transmission, cognitive deficits, and neurodevelopmental abnormalities. Researchers also utilize these antibodies to explore mechanisms of synaptic remodeling during development or injury recovery.
Commercial DLG4 antibodies are typically developed in rabbits or mice, validated for specificity using knockout controls. Their applications span basic neurobiology, drug discovery, and diagnostic research, providing insights into synaptic health and disease pathways.
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