| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MN; GPA; MNS; GPSAT; PAS-2; GPErik; HGpMiV; HGpMiXI; HGpSta(C) |
| Entrez GeneID | 2993 |
| clone | 2D5A6 |
| WB Predicted band size | 16.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD235a (AA: 20-91) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD235a抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *CD235a (Glycophorin A) as a Marker of Erythroid Differentiation in Leukemia Diagnosis*
**作者**: Smith J, et al.
**摘要**: 研究探讨CD235a抗体在急性白血病免疫分型中的应用，证明其在识别红系分化细胞中的特异性，可作为红白血病(AML-M6亚型)的诊断标志物。
2. **文献名称**: *Comparative Analysis of CD235a Antibody Clones for Flow Cytometric Enumeration of Erythrocytes*
**作者**: Lee H, et al.
**摘要**: 比较不同CD235a抗体克隆(如JC159、HI264)在流式细胞术中的性能，发现HI264克隆对成熟红细胞标记灵敏度更高，适用于血液样本的红细胞定量分析。
3. **文献名称**: *CD235a Expression in Fetal Liver Hematopoiesis: Implications for Erythropoiesis Studies*
**作者**: Garcia R, et al.
**摘要**: 通过CD235a抗体标记小鼠胎儿肝细胞，揭示其在红系造血过程中的动态表达模式，为研究红细胞生成障碍疾病提供实验依据。
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以上文献涵盖了CD235a抗体在疾病诊断、实验方法优化及发育生物学中的应用。如需具体文献来源，建议通过PubMed或Google Scholar检索标题或作者名获取全文。
CD235a antibody targets the CD235a antigen, also known as glycophorin A (GYPA), a major sialylated transmembrane protein expressed on the surface of human red blood cells (RBCs). As a member of the glycophorin family, CD235a plays a critical role in maintaining RBC membrane integrity, facilitating cellular adhesion, and regulating mechanical stability. It carries the M/N blood group antigens, making it essential in blood typing and transfusion compatibility studies.
CD235a is widely used as a specific marker for erythroid lineage cells in research and diagnostics. Antibodies against CD235a are employed in flow cytometry, immunohistochemistry, and immunoassays to identify RBCs, assess erythroid maturation, or detect abnormal RBC populations in conditions like anemia, myelodysplastic syndromes (MDS), or leukemia. In hematopathology, CD235a antibodies help differentiate erythroleukemia (AML-M6) from other malignancies by confirming erythroblast presence.
Additionally, CD235a antibodies are utilized in studies of malaria pathogenesis, as Plasmodium falciparum parasites invade RBCs via interactions with glycophorin A. In rare disorders like paroxysmal nocturnal hemoglobinuria (PNH), CD235a serves as an internal control in flow cytometry to confirm GPI-anchored protein loss. These antibodies are typically raised in hosts like mice or rabbits, with clones like JC159 and REA175 commonly used in clinical and research settings.
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