| WB | 1/500 - 1/2000 | Human, Monkey |
| IF | 咨询技术 | Human, Monkey |
| IHC | 1/200 - 1/1000 | Human, Monkey |
| ICC | 技术咨询 | Human, Monkey |
| FCM | 1/200 - 1/400 | Human, Monkey |
| Elisa | 1/10000 | Human, Monkey |
| Aliases | RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52 |
| Entrez GeneID | 3308 |
| clone | 2E4F10H7 |
| WB Predicted band size | 94.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Monkey |
| Immunogen | Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于HSP70抗体的代表性文献(内容为模拟示例，非真实文献)：
1. **《HSP70 as a biomarker for oxidative stress in cancer cells》**
作者：Smith J. et al.
摘要：研究验证了HSP70抗体在检测肿瘤细胞内氧化应激反应中的应用，发现HSP70高表达与乳腺癌化疗耐药性相关。
2. **《Monoclonal antibody targeting HSP70 inhibits neuroinflammation in Alzheimer's models》**
作者：Chen L. et al.
摘要：开发了一种特异性识别HSP70的单克隆抗体，证明其可通过抑制小胶质细胞活化减轻阿尔茨海默病模型中的神经炎症。
3. **《A comparative study of commercial HSP70 antibodies in immunohistochemistry》**
作者：Garcia R. et al.
摘要：系统性比较了6种市售HSP70抗体的灵敏度和特异性，为不同实验场景下的抗体选择提供了指导建议。
4. **《HSP70 antibody-based capture assay detects extracellular vesicles in sepsis》**
作者：Wang Y. et al.
摘要：利用HSP70抗体建立了一种新型细胞外囊泡富集技术，揭示了脓毒症患者血浆中HSP70阳性囊泡与器官衰竭的相关性。
**Background of HSP70 Antibodies**
Heat shock protein 70 (HSP70) is a highly conserved molecular chaperone critical for cellular stress responses, aiding in protein folding, preventing aggregation, and facilitating degradation of misfolded proteins. It is constitutively expressed under normal conditions but upregulated during stress (e.g., heat, hypoxia, toxins). HSP70 plays roles in apoptosis regulation, immune response modulation, and cellular homeostasis.
HSP70 antibodies are immunological tools designed to detect and quantify HSP70 expression in research and diagnostics. These antibodies target specific epitopes of HSP70 isoforms (e.g., HSPA1A, HSPA8) and are used in techniques like Western blotting, immunohistochemistry, and flow cytometry. Their applications span cancer research (HSP70 overexpression in tumors), neurodegenerative diseases (e.g., Alzheimer’s, where protein misfolding occurs), and infectious diseases (pathogen-host interactions).
Commercial HSP70 antibodies are often raised in rabbits or mice using recombinant proteins or synthetic peptides. Validation includes testing specificity against knockout cells or tissues. Cross-reactivity with homologous proteins (e.g., HSP70 family members) requires careful control.
HSP70’s dual role as a cytoprotective agent and a potential disease biomarker makes its study vital. Antibodies enable exploration of its therapeutic potential—for instance, targeting HSP70 in cancer or enhancing its activity in proteinopathies. Their utility in diagnostic assays also underscores HSP70’s relevance in clinical contexts, such as stress response monitoring or prognostic evaluations.
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