| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DSI,GIT, PDI,PHDB, PDIA1,PO4DB, PO4HB,PROHB,CLCRP1, ERBA2L,P4Hbeta |
| Entrez GeneID | 5034 |
| clone | 3F12C11D2 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于P4HB抗体的参考文献概览：
1. **文献名称**: "Prolyl 4-hydroxylase subunit beta (P4HB) promotes colorectal cancer progression through epithelial-mesenchymal transition"
**作者**: Zhang Y, et al.
**摘要**: 研究利用P4HB抗体验证其在结直肠癌中的高表达，发现P4HB通过激活EMT通路促进肿瘤转移，提示其作为癌症治疗靶点的潜力。
2. **文献名称**: "P4HB Silencing Suppresses Progression of Hepatocellular Carcinoma via Endoplasmic Reticulum Stress Signaling"
**作者**: Li X, et al.
**摘要**: 通过P4HB抗体检测肝癌组织中蛋白水平，证实抑制P4HB可诱导内质网应激相关凋亡，抑制肿瘤生长，为肝癌治疗提供新机制。
3. **文献名称**: "Immunohistochemical Detection of P4HB in Lung Fibrosis: Correlation with Disease Severity"
**作者**: Wang H, et al.
**摘要**: 使用P4HB抗体对肺纤维化患者组织染色，发现其表达水平与纤维化程度正相关，提示P4HB可能作为肺纤维化生物标志物。
4. **文献名称**: "P4HB Interacts with HER2 to Promote Breast Cancer Metastasis through MAPK Signaling"
**作者**: Chen L, et al.
**摘要**: 基于P4HB抗体的免疫共沉淀实验发现P4HB与HER2相互作用激活MAPK通路，促进乳腺癌侵袭转移，揭示了新的治疗靶向路径。
注：上述文献信息为示例性概括，实际文献需通过PubMed或Web of Science等平台检索确认。
**Background of P4HB Antibody**
The P4HB (Prolyl 4-hydroxylase subunit beta) protein, also known as Protein Disulfide Isomerase (PDI), is a multifunctional enzyme encoded by the *P4HB* gene. It resides primarily in the endoplasmic reticulum (ER) and plays a critical role in post-translational protein modification. P4HB is a member of the thioredoxin superfamily and functions as both a subunit of the prolyl 4-hydroxylase complex—essential for collagen biosynthesis by catalyzing proline hydroxylation—and as a disulfide isomerase, facilitating the proper folding of nascent polypeptides through oxidation, reduction, and rearrangement of disulfide bonds.
P4HB is implicated in various cellular processes, including ER stress response, cell adhesion, and apoptosis. Its dysregulation has been linked to pathological conditions such as cancer, neurodegenerative diseases (e.g., Alzheimer’s), fibrosis, and ischemic injury. Overexpression of P4HB is observed in tumors, where it promotes cancer cell survival, metastasis, and chemoresistance by mitigating ER stress and enhancing protein folding capacity.
Antibodies targeting P4HB are widely used in research to study its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These tools are vital for exploring P4HB’s role as a potential therapeutic target or biomarker in diseases characterized by protein misfolding or oxidative stress. Commercial P4HB antibodies are typically validated for specificity against human, mouse, or rat samples, with molecular weights around 55-57 kDa.
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