| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DSI,GIT, PDI,PHDB, PDIA1,PO4DB, PO4HB,PROHB,CLCRP1, ERBA2L,P4Hbeta |
| Entrez GeneID | 5034 |
| clone | 4E10C6F6 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于P4HB抗体的3篇代表性文献的简要总结(基于公开信息模拟，非真实文献)：
1. **文献名称**：*Prolyl 4-hydroxylase subunit P4HB: A novel biomarker for tumor progression in colorectal cancer*
**作者**：Li X, et al.
**摘要**：研究通过免疫组化(使用P4HB抗体)发现P4HB在结直肠癌组织中高表达，并与肿瘤转移、患者预后不良相关，提示其作为癌症治疗靶点的潜力。
2. **文献名称**：*P4HB modulates ER stress and autophagy in neurodegenerative diseases: Insights from mouse models*
**作者**：Smith J, et al.
**摘要**：利用P4HB抗体进行Western blot和免疫荧光分析，发现P4HB通过调控内质网应激和自噬通路，影响阿尔茨海默病模型小鼠的神经元存活。
3. **文献名称**：*Antibody-based profiling of P4HB in liver fibrosis: Implications for therapeutic targeting*
**作者**：Wang Y, et al.
**摘要**：通过P4HB抗体检测肝纤维化患者样本，证实P4HB在活化的肝星状细胞中特异性上调，靶向抑制P4HB可减轻胶原沉积和纤维化进展。
如需具体文献，建议在PubMed或Web of Science中以“P4HB antibody” + 研究领域(如癌症、纤维化等)为关键词检索。
The P4HB antibody targets the protein Prolyl 4-Hydroxylase Beta Polypeptide (P4HB), also known as Protein Disulfide Isomerase (PDI), a multifunctional enzyme critical for protein folding and post-translational modifications. P4HB resides in the endoplasmic reticulum (ER) and catalyzes the formation, reduction, and isomerization of disulfide bonds in nascent polypeptides, ensuring proper tertiary structure. It also acts as a chaperone, assisting in collagen biosynthesis by serving as the beta-subunit of prolyl 4-hydroxylase, which hydroxylates proline residues in collagen precursors—a key step for collagen stability.
P4HB is implicated in diverse physiological and pathological processes, including cancer progression, neurodegenerative disorders, and cardiovascular diseases. Overexpression of P4HB has been observed in various tumors, correlating with metastasis, drug resistance, and poor prognosis, likely due to its role in mitigating ER stress and supporting cancer cell survival. In Alzheimer’s disease, P4HB interacts with tau protein, potentially influencing neurofibrillary tangle formation.
Antibodies against P4HB are widely used in research to study its expression, localization, and function. They aid in detecting P4HB levels in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercially available P4HB antibodies include monoclonal and polyclonal variants, often raised in rabbits or mice. Researchers also utilize these antibodies to explore P4HB's therapeutic potential, as its inhibition may disrupt pathological processes like fibrosis or tumor growth. Understanding P4HB's regulatory mechanisms remains vital for developing targeted therapies in multiple diseases.
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