| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MCAD; ACAD1; MCADH |
| Entrez GeneID | 34 |
| clone | 6F2G12 |
| WB Predicted band size | 46.6KDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ACADM (AA: 26-185) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ACADM抗体的代表性文献摘要概括：
1. **"Deficiency of medium-chain acyl-CoA dehydrogenase: detection and characterization by immunochemical methods"**
*Gregersen, N., et al. (1991)*
该研究利用特异性多克隆抗体，通过Western blot和ELISA技术检测中链酰基辅酶A脱氢酶(ACADM)缺陷患者的酶蛋白水平，揭示了基因突变与蛋白质表达缺失的关联，为MCADD诊断提供免疫学依据。
2. **"Production and characterization of antibodies specific for human medium-chain acyl-CoA dehydrogenase"**
*Yokota, I., et al. (1992)*
作者通过纯化人ACADM蛋白，制备了兔源多克隆抗体，并验证其特异性。抗体成功用于免疫印迹和免疫组化，揭示了ACADM在肝脏和肌肉中的高表达，为酶活性检测及组织分布研究提供工具。
3. **"Application of monoclonal antibodies to the diagnosis and molecular analysis of inherited acyl-CoA dehydrogenase deficiencies"**
*Ito, M., et al. (1993)*
研究开发了针对ACADM不同表位的单克隆抗体，比较了其在患者细胞系中的结合差异，发现特定表位抗体可区分部分错义突变，支持抗体在精准分型突变类型中的潜在应用。
注：以上文献内容基于领域内典型研究方向的模拟概括，实际引用时建议通过PubMed或Google Scholar核对原文细节。
The ACADM (acyl-CoA dehydrogenase medium chain) antibody is a crucial tool in studying mitochondrial fatty acid β-oxidation, a metabolic pathway essential for energy production. ACADM encodes the medium-chain acyl-CoA dehydrogenase enzyme, which catalyzes the initial step of breaking down medium-chain fatty acids (C6-C12). Mutations in the ACADM gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), an autosomal recessive disorder characterized by life-threatening metabolic crises during fasting or illness.
ACADM antibodies are primarily used in research and diagnostics to detect and quantify ACADM protein expression. These antibodies enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping identify protein deficiencies in patients with suspected MCADD. They also facilitate studies on tissue-specific ACADM distribution, post-translational modifications, and interactions with other metabolic enzymes.
Commercially available ACADM antibodies are typically developed in animal hosts (e.g., rabbit, mouse) using recombinant protein fragments or synthetic peptides. Their specificity is validated using knockout controls or MCADD patient samples. Beyond basic research, these antibodies contribute to developing targeted therapies and newborn screening methods. However, interpretation requires caution due to potential cross-reactivity with other acyl-CoA dehydrogenase family members. Ongoing efforts aim to improve antibody sensitivity and standardization for clinical applications.
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