| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HO-1; HSP32; HMOX1D; bK286B10 |
| Entrez GeneID | 3162 |
| clone | 2D5E9 |
| WB Predicted band size | 32.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HMOX1 (AA: 1-110) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HMOX1抗体的3-4篇参考文献的简要概括：
1. **文献名称**：*Heme oxygenase-1: function, regulation, and implication of a novel stress-inducible protein in oxidant-induced lung injury*
**作者**：Choi AM, Alam J
**摘要**：该综述总结了HMOX1(HO-1)在氧化应激中的关键作用，并提到利用特异性抗体在肺损伤模型中检测其表达上调，揭示了其在细胞保护中的机制。
2. **文献名称**：*Role of heme oxygenase-1 in cardiovascular function and disease*
**作者**：Duckers HJ, Boehm M, True AL, et al.
**摘要**：研究通过免疫组化(使用HMOX1抗体)发现HO-1在动脉粥样硬化斑块中的表达与炎症缓解相关，表明其作为心血管疾病治疗靶点的潜力。
3. **文献名称**：*Curcumin induces heme oxygenase-1 through a calcium-independent pathway*
**作者**：Motterlini R, Foresti R, Bassi R, et al.
**摘要**：文章通过Western blot和免疫荧光技术(依赖HMOX1抗体)证实姜黄素通过非钙依赖途径诱导HO-1表达，为其抗氧化机制提供依据。
4. **文献名称**：*Heme oxygenase-1-derived carbon monoxide suppresses LPS-induced endothelial inflammation*
**作者**：Wagener FA, Volk HD, Willis D, et al.
**摘要**：研究使用HMOX1抗体阻断实验，发现HO-1通过产生CO抑制内皮细胞炎症反应，强调了其在抗炎通路中的功能。
这些文献均通过HMOX1抗体在蛋白水平检测、定位或功能研究中验证了其作用，涵盖疾病模型、药物机制及信号通路探索。
The HMOX1 antibody is a crucial tool in biomedical research for detecting heme oxygenase 1 (HO-1), an enzyme encoded by the HMOX1 gene. HO-1 catalyzes the breakdown of heme into carbon monoxide, free iron, and biliverdin, which is subsequently converted to bilirubin. These byproducts possess antioxidant, anti-inflammatory, and cytoprotective properties, making HO-1 a key player in cellular defense against oxidative stress, inflammation, and tissue injury. HMOX1 expression is tightly regulated and induced by various stressors, including oxidative damage, heavy metals, UV radiation, and inflammatory cytokines.
HMOX1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study HO-1's expression, localization, and regulation in tissues and cells. They enable researchers to investigate its role in diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer's and Parkinson's), cardiovascular conditions, and diabetes. In cancer, HO-1 exhibits dual roles, either promoting tumor progression by aiding cancer cell survival or suppressing malignancy through anti-inflammatory effects. The antibody also aids in exploring therapeutic strategies targeting HO-1 pathways, including its potential in organ transplantation and ischemia-reperfusion injury. Available as monoclonal or polyclonal variants, HMOX1 antibodies are essential for advancing understanding of cellular stress responses and developing HO-1-based interventions.
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