WB | 1/500 - 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
ICC | 1/100 - 1/500 | Human,Mouse,Rat |
FCM | 1/200 - 1/400 | Human,Mouse,Rat |
Elisa | 1/10000 | Human,Mouse,Rat |
Aliases | TCRB; TCRBC1; BV05S1J2.2 |
Entrez GeneID | 28639 |
clone | 5D9F1 |
WB Predicted band size | 19.7kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Rat |
Immunogen | Purified recombinant fragment of human TRBC1 (AA: 1-149) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TRBC1抗体的3篇参考文献摘要(信息基于公开研究整理,非真实文献,仅供示例参考):
1. **文献名称**: "TRBC1 as a Biomarker for Clonal T-Cell Populations"
**作者**: Smith A, et al.
**摘要**: 本研究验证了TRBC1抗体在流式细胞术中的应用,证明其可通过检测T细胞受体β链恒定区的表达差异,有效区分单克隆与多克隆T细胞增殖,为T细胞淋巴瘤的诊断提供新方法。
2. **文献名称**: "Development of a TRBC1-Specific Monoclonal Antibody for Immunoassays"
**作者**: Chen L, et al.
**摘要**: 报道了一种新型TRBC1单克隆抗体的开发与验证,该抗体在免疫组化中表现出高特异性,可辅助鉴别正常T细胞与恶性T细胞克隆,提升病理诊断准确性。
3. **文献名称**: "Targeting TRBC1 in T-Cell Malignancies: Diagnostic and Therapeutic Implications"
**作者**: García-Muñoz R, et al.
**摘要**: 探讨TRBC1在T细胞肿瘤中的生物学意义,发现其抗体不仅可用于微小残留病监测,还可能通过靶向治疗策略抑制T细胞恶性肿瘤进展。
(注:以上为模拟内容,实际文献需通过PubMed/Google Scholar等平台以“TRBC1 antibody”、“T-cell receptor beta constant”为关键词检索获取。)
TRBC1 (T-cell receptor beta constant region 1) is a component of the T-cell receptor (TCR) complex, which plays a central role in adaptive immunity by recognizing antigenic peptides presented by major histocompatibility complex (MHC) molecules. The TCR β-chain consists of variable (V), diversity (D), joining (J), and constant (C) regions. TRBC1 encodes one of two conserved constant regions (TRBC1 and TRBC2) expressed in the β-chain, with TRBC1 present in approximately 65-70% of mature αβ T-cells. This dichotomy allows TRBC1-specific antibodies to distinguish monoclonal from polyclonal T-cell populations, as normal T-cells exhibit a balanced TRBC1/TRBC2 distribution, while clonal T-cell malignancies (e.g., T-cell lymphomas or leukemias) typically express only one isoform.
TRBC1 antibodies are valuable tools in research and diagnostics. They enable the detection of clonal TCR β-chain expression via flow cytometry or immunohistochemistry, aiding in the diagnosis and classification of T-cell neoplasms. For example, TRBC1 staining can help differentiate reactive T-cell expansions (polyclonal, mixed TRBC1/TRBC2) from malignant clones (monoclonal, TRBC1+ or TRBC1-). Recent advancements have also explored TRBC1 as a potential therapeutic target, particularly in chimeric antigen receptor (CAR) T-cell therapies for TRBC1-restricted malignancies. However, cross-reactivity and isoform specificity remain critical considerations in assay design and clinical applications. Overall, TRBC1 antibodies contribute significantly to understanding T-cell biology and improving the management of T-cell-related disorders.
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