| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BLR2; EBI1; CCR-7; CCR7; CDw197; CMKBR7; CC-CKR-7 |
| Entrez GeneID | 1236 |
| clone | 4B7B8 |
| WB Predicted band size | 42.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD197 (AA: extra mix) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇涉及NEDD9抗体的代表性文献，供参考：
1. **文献名称**：*NEDD9 promotes invasive behavior in colorectal cancer*
**作者**：Kim JC, et al.
**摘要**：研究使用NEDD9抗体通过Western blot和免疫组化分析结直肠癌组织中NEDD9的表达，发现其高表达与肿瘤侵袭性增强相关，并激活下游FAK/Src信号通路。
2. **文献名称**：*NEDD9 regulates actin dynamics through collagen-induced Src/FAK activation*
**作者**：Izumchenko E, et al.
**摘要**：利用NEDD9抗体进行免疫沉淀和共聚焦显微镜观察，证明NEDD9通过整合素介导的细胞骨架重塑促进肿瘤细胞迁移，并依赖Src激酶活性。
3. **文献名称**：*NEDD9 is a novel regulator of epithelial-mesenchymal transition in melanoma*
**作者**：Natarajan M, et al.
**摘要**：通过NEDD9抗体检测黑色素瘤细胞系中的表达变化，发现NEDD9通过调控Snail和E-cadherin表达驱动EMT过程，增强转移潜能。
4. **文献名称**：*NEDD9 interacts with viral oncoproteins to promote cell cycle progression*
**作者**：Khan AQ, et al.
**摘要**：研究利用NEDD9抗体进行免疫共沉淀，揭示其与HPV E6蛋白的相互作用，导致细胞周期蛋白D1上调，促进病毒诱导的肿瘤发生。
---
以上文献均涉及NEDD9抗体的实验应用(如WB、IP、IHC等)，并聚焦于其在肿瘤转移、信号通路及病毒感染中的作用。如需具体DOI或年份可进一步补充。
The NEDD9 (Neural precursor cell expressed, developmentally down-regulated 9) protein, also known as HEF1 or Cas-L, is a scaffolding adaptor protein belonging to the Cas (Crk-associated substrate) family. It plays critical roles in regulating cell adhesion, migration, and invasion by integrating signals from integrins, growth factor receptors, and other extracellular cues. Structurally, NEDD9 contains multiple protein interaction domains, including SH3-binding motifs and tyrosine phosphorylation sites, enabling its participation in pathways like FAK/Src, RAS-MAPK, and TGF-β signaling. Dysregulation of NEDD9 is linked to cancer progression, particularly in promoting metastasis in melanoma, breast, and lung cancers.
NEDD9 antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). High-quality NEDD9 antibodies specifically recognize epitopes within its N-terminal domain or C-terminal region, enabling detection of full-length proteins or phosphorylation-dependent activation states. Commercial NEDD9 antibodies are available in various host species (e.g., rabbit monoclonal, mouse polyclonal) and are validated for cross-reactivity in human, mouse, and rat samples. Research utilizing these antibodies has advanced understanding of NEDD9's role in EMT (epithelial-mesenchymal transition), cytoskeletal dynamics, and therapeutic resistance, making it a biomarker of interest in oncology and cell biology.
×
