| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DSI,GIT, PDI,PHDB, PDIA1,PO4DB, PO4HB,PROHB,CLCRP1, ERBA2L,P4Hbeta |
| Entrez GeneID | 5034 |
| clone | 5D12A4E5 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于P4HB抗体的参考文献概览(文献信息为模拟示例，实际需根据具体文献调整)：
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1. **文献名称**: *P4HB promotes hepatocellular carcinoma progression through ER stress-mediated autophagy*
**作者**: Zhang L, et al. (2020)
**摘要**: 研究利用P4HB抗体检测其在肝癌组织中的高表达，发现P4HB通过内质网应激-自噬轴促进肿瘤侵袭，提示其作为肝癌治疗靶点的潜力。
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2. **文献名称**: *Prolyl 4-hydroxylase subunit beta (P4HB) as a novel biomarker for pulmonary fibrosis*
**作者**: Kim S, et al. (2019)
**摘要**: 通过P4HB抗体染色发现，肺纤维化患者肺组织中P4HB显著上调，其通过调控胶原羟基化促进纤维化进程，为疾病诊断提供新标志物。
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3. **文献名称**: *P4HB interacts with HER2 to drive breast cancer metastasis via integrin signaling*
**作者**: Wang Y, et al. (2021)
**摘要**: 采用P4HB抗体进行免疫共沉淀实验，揭示P4HB与HER2蛋白互作，激活整合素通路促进乳腺癌转移，为联合靶向治疗提供依据。
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**提示**：实际文献需通过PubMed/Google Scholar检索关键词“P4HB antibody”、“P4HB cancer/therapy”等获取。建议关注抗体应用场景(如Western Blot、IHC)及疾病机制研究方向的论文。
The P4HB (Prolyl 4-Hydroxylase Beta Subunit) antibody is a tool used to detect the P4HB protein, a key player in cellular protein modification and quality control. P4HB, also known as Protein Disulfide Isomerase (PDI), is an endoplasmic reticulum (ER)-resident enzyme critical for catalyzing the formation, breakage, and rearrangement of disulfide bonds during protein folding. It ensures proper tertiary structure and stability of nascent proteins, particularly secretory and membrane proteins. Additionally, P4HB acts as a chaperone, aiding in the assembly of collagen molecules by hydroxylating proline residues, a step essential for collagen triple-helix formation.
Beyond its role in normal physiology, P4HB is implicated in various diseases. Overexpression has been observed in cancers, where it supports tumor progression by enhancing cell survival under hypoxic or ER-stress conditions. It also contributes to fibrotic disorders by promoting collagen deposition. Researchers use P4HB antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its expression patterns, subcellular localization, and interactions in disease models. These studies aim to explore P4HB’s potential as a therapeutic target or biomarker for conditions linked to protein misfolding, oxidative stress, or aberrant collagen synthesis. The antibody’s specificity and validation across species and applications make it valuable in both basic research and clinical investigations.
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