Mouse Monoclonal ETFA Antibody

The ETFA antibody is a crucial tool for studying the electron transfer flavoprotein alpha subunit (ETFA), a key component in mitochondrial energy metabolism. ETFA, along with ETFB, forms the heterodimeric electron transfer flavoprotein (ETF), which acts as an electron shuttle between primary dehydrogenases (e.g., fatty acyl-CoA dehydrogenases) and the membrane-bound ETF-ubiquinone oxidoreductase (ETF-QO) in the mitochondrial matrix. This system is essential for fatty acid β-oxidation and amino acid catabolism. Mutations in the ETFA gene are linked to glutaric aciduria type II (GA-II), a rare autosomal recessive disorder characterized by metabolic acidosis, hypoglycemia, and cardiomyopathy. ETFA antibodies, typically generated in rabbits or mice using recombinant or peptide antigens, enable the detection and quantification of ETFA expression in tissues or cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They are vital for investigating ETFA dysfunction in metabolic diseases, validating gene-editing models, and exploring therapeutic strategies. Commercial ETFA antibodies are often validated for specificity and sensitivity, with applications spanning basic research, clinical diagnostics, and drug development. Their use has advanced our understanding of mitochondrial disorders and cellular energy regulation.