| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EMA; GA2; MADD |
| Entrez GeneID | 2108 |
| clone | 2F8A5 |
| WB Predicted band size | 35kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ETFA抗体的参考文献示例(注：以下内容为模拟虚构，仅作参考格式示例)：
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1. **文献名称**: "Deficiency of Electron Transfer Flavoprotein Alpha (ETFA) Identified via Immunoblotting in a Patient with Glutaric Acidemia Type II"
**作者**: Smith, J.R., et al.
**摘要**: 本研究通过Western Blot技术结合特异性ETFA抗体，在一名Ⅱ型戊二酸血症患者中发现ETFA蛋白表达缺失，揭示了ETFA基因突变与线粒体脂肪酸氧化障碍的关联。
2. **文献名称**: "Localization of ETFA in Mouse Skeletal Muscle Using Immunofluorescence: Implications for Metabolic Myopathies"
**作者**: Tanaka, M., & Yamamoto, S.
**摘要**: 利用ETFA抗体进行免疫荧光染色，发现ETFA蛋白在小鼠骨骼肌线粒体中的特异性定位，为研究代谢性肌病中线粒体功能异常提供了形态学依据。
3. **文献名称**: "Development and Validation of a Novel ETFA Monoclonal Antibody for Diagnostic Applications"
**作者**: Chen, L., et al.
**摘要**: 文章报道了一种高亲和力ETFA单克隆抗体的开发，并通过ELISA和免疫组化验证其在诊断遗传性代谢疾病中的灵敏度和特异性，为临床检测提供新工具。
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*提示*：如需真实文献，建议通过PubMed或Google Scholar检索关键词“ETFA antibody”、“Electron Transfer Flavoprotein Alpha”+“antibody application”。实际研究中，ETFA抗体常用于代谢疾病诊断(如多种酰基辅酶A脱氢酶缺乏症，MADD)及线粒体功能研究。
The ETFA antibody targets the alpha subunit of the Electron Transfer Flavoprotein (ETF), a critical component in mitochondrial energy metabolism. ETF, composed of alpha (ETFA) and beta (ETFB) subunits, acts as an electron carrier in fatty acid β-oxidation and amino acid catabolism. It shuttles electrons from acyl-CoA dehydrogenases to the respiratory chain via ETF-ubiquinone oxidoreductase (ETF-QO). ETFA mutations are linked to genetic disorders like glutaric acidemia type II (GA-II), a rare autosomal recessive condition characterized by metabolic acidosis, hypoglycemia, and cardiomyopathy.
ETFA antibodies are essential tools in research to study ETF expression, localization, and function in cellular models or tissues. They are used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in metabolic studies or disease models. Researchers also employ these antibodies to investigate the molecular mechanisms underlying GA-II and other mitochondrial disorders, aiding in diagnostic approaches or therapeutic development.
Commercial ETFA antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Specific validation steps (e.g., knockout controls) are crucial to ensure antibody reliability. Understanding ETFA's role and its antibody applications enhances insights into mitochondrial dysfunction and potential treatments for metabolic diseases.
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