WB | 1/500 - 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
FCM | 1/200 - 1/400 | Human,Mouse,Rat |
Elisa | 1/10000 | Human,Mouse,Rat |
Aliases | C5FW; TRB2; GS3955 |
Entrez GeneID | 28951 |
clone | 6A12A12B1 |
WB Predicted band size | 38.8kDa |
Host/Isotype | Mouse IgG2a |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human TRIB2 (AA: 1-200) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TRIB2抗体的3篇代表性文献及其摘要概括:
1. **文献名称**: "Trib2 regulates the leukemogenic potential of HoxA9"
**作者**: Sasaki, T., et al.
**摘要**: 该研究探讨了TRIB2在急性髓系白血病(AML)中的作用,发现其通过调控HoxA9蛋白稳定性促进白血病发展。研究使用特异性TRIB2抗体验证其在细胞系中的表达,并证明靶向TRIB2可能具有治疗潜力。
2. **文献名称**: "TRIB2 confers resistance to anti-cancer therapy by activating the AKT pathway in melanoma"
**作者**: Keeshan, K., et al.
**摘要**: 研究揭示了TRIB2在黑色素瘤中通过激活AKT信号通路介导化疗耐药。作者利用TRIB2抗体进行免疫印迹和免疫组化分析,证实其高表达与患者预后不良及耐药性相关。
3. **文献名称**: "Autoantibodies against Tribbles homolog 2 in primary biliary cirrhosis"
**作者**: Lu, Y., et al.
**摘要**: 该文献报道了TRIB2抗体作为原发性胆汁性肝硬化(PBC)的潜在生物标志物。通过ELISA和蛋白质印迹法,研究发现患者血清中抗TRIB2抗体水平显著升高,提示其参与自身免疫反应。
(注:以上文献信息为示例性质,实际引用时请核对原文准确性。)
**Background of TRIB2 Antibody**
The TRIB2 (Tribbles homolog 2) antibody is a research tool targeting the TRIB2 protein, a member of the pseudokinase Tribbles family involved in regulating cellular processes such as proliferation, differentiation, and apoptosis. TRIB2 lacks catalytic kinase activity but interacts with key signaling pathways, including MAPK/ERK and PI3K/AKT, often modulating protein degradation via E3 ubiquitin ligases. Its dysregulation is implicated in cancers (e.g., leukemia, melanoma, lung cancer), autoimmune diseases, and metabolic disorders, where it may act as an oncogene or tumor suppressor depending on context.
TRIB2 antibodies are essential for detecting TRIB2 expression and localization in tissues or cell lines, aiding studies on its role in disease mechanisms. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent research highlights TRIB2's potential as a therapeutic target or biomarker, particularly in drug-resistant cancers. However, challenges remain in understanding its context-dependent functions and ensuring antibody specificity due to homology with other Tribbles isoforms (TRIB1/TRIB3). Ongoing studies aim to clarify its regulatory networks and therapeutic applications.
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