WB | 1/500 - 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
FCM | 1/200 - 1/400 | Human,Mouse,Rat |
Elisa | 1/10000 | Human,Mouse,Rat |
Aliases | ARHA; ARH12; RHO12; EDFAOB; RHOH12 |
Entrez GeneID | 387 |
clone | 7E7H4 |
WB Predicted band size | 22kDa |
Host/Isotype | Mouse IgG2b |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human RHOA (AA: 1-190) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RHOA抗体的3篇参考文献示例(注:内容为模拟概括,非真实文献):
1. **文献名称**:*RhoA-specific antibodies reveal the dynamics of cytoskeletal reorganization in cancer cell migration*
**作者**:Haga, R.B., et al.
**摘要**:该研究利用RHOA特异性抗体,揭示了RHOA在癌细胞迁移过程中通过调控肌动蛋白应力纤维的形成,促进肿瘤侵袭的分子机制,为靶向RhoA信号通路提供了实验依据。
2. **文献名称**:*Autoantibodies against RhoA in autoimmune diseases: Diagnostic potential and functional implications*
**作者**:Suzuki, T., et al.
**摘要**:作者发现系统性硬化症患者血清中存在RHOA自身抗体,并通过体外实验证明这些抗体可异常激活RhoA信号通路,导致成纤维细胞过度活化,提示其可能参与纤维化病理过程。
3. **文献名称**:*RhoA antibody-based inhibition of vascular smooth muscle contraction*
**作者**:Yamamoto, K., et al.
**摘要**:研究使用RHOA中和抗体阻断血管平滑肌细胞中RhoA/ROCK通路,显著抑制钙离子敏感性收缩反应,为高血压和血管痉挛性疾病提供了潜在治疗策略。
如需具体文献,建议通过PubMed或Web of Science以“RHOA antibody”为关键词检索最新研究。
**Background of RHOA Antibodies**
RhoA (Ras homolog family member A) is a small GTPase protein regulating critical cellular processes like cytoskeleton dynamics, cell migration, proliferation, and gene expression. Dysregulation of RhoA signaling is implicated in cancer, autoimmune disorders, and cardiovascular diseases. RHOA antibodies are immunological tools designed to detect and study RhoA expression, localization, and activity in research or diagnostic contexts.
In cancer, RHOA mutations or altered expression are linked to tumor progression, metastasis, and drug resistance. For example, RHOA hyperactivity is observed in gastric, breast, and lung cancers. Antibodies targeting RhoA help identify its role in oncogenic pathways or tumor microenvironment interactions. In autoimmune diseases like rheumatoid arthritis, aberrant RhoA signaling contributes to inflammation and tissue damage; antibodies aid in mapping these mechanisms.
Therapeutic applications are emerging, with RHOA antibodies explored as biomarkers for disease prognosis or treatment response. Challenges include ensuring specificity due to RhoA's structural similarity to other Rho GTPases and variability in post-translational modifications. Additionally, RHOA antibodies are used in drug development, such as screening inhibitors targeting RhoA-driven pathways.
Overall, RHOA antibodies serve as vital reagents for unraveling RhoA's pathophysiological roles and advancing targeted therapies, though standardization and validation remain critical for clinical translation.
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