| WB | 1/500 - 1/2000 | Human, Mouse, Rat, Monkey |
| IF | 咨询技术 | Human, Mouse, Rat, Monkey |
| IHC | 1/200 - 1/1000 | Human, Mouse, Rat, Monkey |
| ICC | 技术咨询 | Human, Mouse, Rat, Monkey |
| FCM | 1/200 - 1/400 | Human, Mouse, Rat, Monkey |
| Elisa | 1/10000 | Human, Mouse, Rat, Monkey |
| Aliases | OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m |
| Entrez GeneID | 498 |
| clone | 3A6B9 |
| WB Predicted band size | 59.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Immunogen | Purified recombinant fragment of human ATP5F1A (AA: 44-220) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"Characterization of a monoclonal antibody against human ATP synthase α subunit (ATP5F1A) and its application in mitochondrial studies"**
- Author: J. M. Smith et al.
- 摘要：该研究开发了一种针对ATP5F1A的单克隆抗体，验证了其在Western blot和免疫荧光中的特异性，并用于分析线粒体功能障碍疾病模型中的蛋白表达变化。
2. **"ATP5F1A as a biomarker in neurodegenerative disorders: Insights from antibody-based assays"**
- Author: L. Chen et al.
- 摘要：通过抗ATP5F1A的抗体检测阿尔茨海默病和帕金森病患者脑组织样本，发现ATP5F1A表达水平与线粒体活性损伤呈负相关，提示其作为疾病生物标志物的潜力。
3. **"Immunohistochemical localization of ATP synthase α subunit in cardiac tissues using a novel polyclonal antibody"**
- Author: R. K. Patel et al.
- 摘要：报道了一种新型多克隆抗体的开发，用于心肌组织中ATP5F1A的定位研究，揭示了其在缺血再灌注损伤模型中的分布变化及功能意义。
4. **"Antibody validation for mitochondrial proteomics: A case study on ATP5F1A in metabolic diseases"**
- Author: S. Müller et al.
- 摘要：系统评估了多种商业ATP5F1A抗体的特异性，结合质谱分析验证其在代谢综合征患者样本中线粒体蛋白质组研究中的可靠性。
(注：上述文献为示例，实际引用需根据真实文献调整。)
ATP5F1A, also known as ATP synthase F1 subunit alpha, is a critical component of the mitochondrial ATP synthase complex (Complex V), which catalyzes ATP production during oxidative phosphorylation. This nuclear-encoded subunit forms the catalytic core of the F1 sector, working in conjunction with the F0 transmembrane domain to generate ATP via a proton gradient across the inner mitochondrial membrane. ATP5F1A antibodies are essential tools for studying the expression, localization, and function of this protein in cellular energy metabolism.
These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess ATP5F1A levels in tissues or cultured cells, particularly in research on mitochondrial disorders, cancer, and neurodegenerative diseases. Dysregulation of ATP5F1A has been linked to conditions such as Leigh syndrome, Alzheimer’s disease, and various cancers, where altered ATP synthesis impacts cell survival and proliferation.
Commercial ATP5F1A antibodies are typically raised against epitopes in conserved regions of the protein, often validated for specificity using knockout controls. Researchers prioritize antibodies with cross-reactivity across species (e.g., human, mouse, rat) for comparative studies. Recent applications include investigating metabolic reprogramming in tumors and evaluating mitochondrial dysfunction in aging. Proper validation and optimization of antibody protocols remain crucial due to potential cross-reactivity with other ATP synthase subunits or isoforms.
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