| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | C5FW; TRB2; GS3955 |
| Entrez GeneID | 28951 |
| clone | 6A12A12 |
| WB Predicted band size | 38.8kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TRIB2 (AA: 1-200) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TRIB2抗体的3篇代表性文献(内容基于公开研究概括，非真实文献，仅供参考)：
1. **文献名称**: "TRIB2 promotes cancer cell proliferation and survival through AKT signaling in acute myeloid leukemia"
**作者**: Smith J.R., et al.
**摘要**: 该研究利用TRIB2特异性抗体，通过Western blot和免疫组化分析发现，TRIB2在急性髓系白血病(AML)中高表达，并通过激活AKT信号通路促进癌细胞存活，抑制凋亡。
2. **文献名称**: "Autoantibodies against TRIB2 in autoimmune diseases: A potential diagnostic biomarker"
**作者**: Chen L., et al.
**摘要**: 研究通过ELISA和蛋白质微阵列技术检测到系统性红斑狼疮(SLE)患者血清中存在针对TRIB2的自身抗体，提示TRIB2抗体可能与自身免疫疾病的病理机制相关，并可能作为诊断标志物。
3. **文献名称**: "Targeting TRIB2 with monoclonal antibodies inhibits tumor growth in colorectal cancer models"
**作者**: Wang Y., et al.
**摘要**: 开发了靶向TRIB2的单克隆抗体，体外和动物实验显示其能特异性结合TRIB2蛋白，抑制结直肠癌细胞增殖并诱导凋亡，为TRIB2抗体在癌症治疗中的应用提供了依据。
(注：以上文献为示例性内容，实际引用请根据具体研究通过PubMed或Google Scholar检索。)
**Background of TRIB2 Antibody**
The TRIB2 (Tribbles homolog 2) antibody is a tool used to detect and study the TRIB2 protein, a member of the pseudokinase Tribbles family. TRIB2 lacks catalytic kinase activity but plays regulatory roles in cell proliferation, differentiation, and apoptosis by interacting with signaling pathways like MAPK, PI3K/AKT, and NF-κB. It is implicated in cancer, where its overexpression is linked to tumor progression, therapy resistance, and poor prognosis in malignancies such as acute myeloid leukemia (AML), lung cancer, and liver cancer. TRIB2 also interacts with transcription factors (e.g., C/EBPα, ATF4) and modulates protein degradation via ubiquitin ligases.
In autoimmune and inflammatory diseases, TRIB2 is associated with autoantibody production in conditions like rheumatoid arthritis and type 1 diabetes. Research using TRIB2 antibodies has advanced understanding of its dual roles in oncogenesis and immune regulation. Commercial TRIB2 antibodies (monoclonal/polyclonal) vary in specificity, requiring validation for applications like Western blot, immunohistochemistry, or flow cytometry. Challenges include cross-reactivity and tissue-specific expression patterns, emphasizing the need for standardized protocols. Overall, TRIB2 antibodies are vital for exploring its function as a biomarker or therapeutic target.
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