| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DSI,GIT,PDI,PHDB,PDIA1,PO4DB,PO4HB,PROHB,CLCRP1,ERBA2L,P4Hbeta |
| Entrez GeneID | 5034 |
| clone | 4E10C6 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P4HB (AA: 309-508) expressed in Mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于P4HB抗体的3篇参考文献示例(文献信息为模拟生成，仅供参考)：
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1. **文献名称**: *P4HB overexpression correlates with poor prognosis in gastric cancer and promotes tumor metastasis*
**作者**: Zhang L, et al.
**摘要**: 本研究通过免疫组化(使用P4HB特异性抗体)发现P4HB在胃癌组织中显著高表达，且与患者生存期缩短及淋巴结转移相关。机制研究表明P4HB通过调控EMT通路促进肿瘤侵袭。
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2. **文献名称**: *Prolyl 4-hydroxylase beta subunit (P4HB) as a novel biomarker in liver fibrosis*
**作者**: Wang Y, et al.
**摘要**: 利用P4HB抗体进行Western blot和免疫荧光分析，证实P4HB在肝纤维化模型小鼠中表达上调，抑制P4HB可减轻胶原沉积，提示其作为肝纤维化治疗靶点的潜力。
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3. **文献名称**: *Antibody-based targeting of P4HB enhances chemosensitivity in colorectal cancer*
**作者**: Kim S, et al.
**摘要**: 研究开发了靶向P4HB的单克隆抗体，体外实验显示该抗体可增强结直肠癌细胞对5-FU的敏感性，机制涉及内质网应激通路激活及凋亡增加。
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**注**：以上文献信息为示例，实际引用时需查询真实数据库(如PubMed、Web of Science)获取准确信息。
The P4HB antibody targets the protein Prolyl 4-Hydroxylase Beta Subunit (P4HB), also known as Protein Disulfide Isomerase A1 (PDIA1), a member of the protein disulfide isomerase (PDI) family. P4HB is a multifunctional enzyme primarily located in the endoplasmic reticulum (ER), where it plays critical roles in protein folding, oxidative maturation, and quality control. It catalyzes the formation and isomerization of disulfide bonds during post-translational modification of nascent polypeptides, ensuring proper tertiary structure. Additionally, P4HB acts as the beta subunit of prolyl 4-hydroxylase, an enzyme essential for collagen biosynthesis by hydroxylating proline residues in procollagen, a step vital for collagen stability and extracellular matrix formation.
P4HB is implicated in various physiological and pathological processes, including ER stress response, tissue fibrosis, and tumor progression. Its overexpression has been observed in cancers, where it supports tumor cell survival, metastasis, and drug resistance by mitigating ER stress and enhancing protein folding capacity. P4HB antibodies are widely used in research to study its expression, localization, and function in diseases. Applications include Western blotting, immunohistochemistry, and immunofluorescence to investigate P4HB's role in cellular stress pathways, collagen metabolism, and cancer biology. These antibodies are valuable tools for understanding mechanisms underlying fibrosis, cancer, and other conditions linked to ER dysfunction or aberrant protein homeostasis.
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