| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | 16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1 |
| Entrez GeneID | 537 |
| clone | 3H11B12 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ATP6AP1抗体的3篇参考文献及其简要摘要：
1. **"ATP6AP1 deficiency impairs autophagosome-lysosome fusion via affecting vesicular translocation during autophagy"**
- 作者：Li Y, et al.
- 摘要：研究利用ATP6AP1抗体进行免疫印迹和免疫荧光实验，发现ATP6AP1缺失通过破坏自噬体-溶酶体间的囊泡转运，导致自噬功能异常，揭示了其在细胞自噬中的关键作用。
2. **"ATP6AP1 modulates immune response in systemic lupus erythematosus by regulating B cell homeostasis"**
- 作者：Wang Q, et al.
- 摘要：通过Western blot和流式细胞术结合ATP6AP1抗体，发现ATP6AP1在系统性红斑狼疮患者B细胞中表达下调，影响B细胞存活和自身抗体产生，提示其与自身免疫疾病的关联。
3. **"Vacuolar ATPase assembly factor ATP6AP1 promotes hepatocellular carcinoma progression via enhancing Notch signaling"**
- 作者：Zhang L, et al.
- 摘要：使用ATP6AP1抗体进行免疫组化分析，证实ATP6AP1在肝癌组织中高表达，并通过激活Notch信号通路促进肿瘤生长和转移，为肝癌治疗提供潜在靶点。
(注：以上文献信息为示例，实际引用时需根据真实文献调整。)
The ATP6AP1 antibody targets ATPase H+ Transporting Accessory Protein 1 (ATP6AP1), a critical subunit of the vacuolar-type H+-ATPase (V-ATPase) complex. V-ATPase is a multi-subunit proton pump responsible for acidifying intracellular compartments, such as lysosomes, endosomes, and secretory vesicles, which is essential for processes like protein degradation, membrane trafficking, and neurotransmitter release. ATP6AP1. also known as Ac45. plays a regulatory role in V-ATPase assembly, localization, and function. Beyond its role in pH regulation, ATP6AP1 has been implicated in cellular signaling pathways, including the Wnt/β-catenin cascade, and is linked to physiological processes such as autophagy and immune response.
Antibodies against ATP6AP1 are widely used in research to study its expression, localization, and interaction partners. They are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore ATP6AP1's involvement in diseases, including cancer, neurodegenerative disorders, and renal tubular acidosis. Dysregulation of ATP6AP1 has been associated with tumor progression, immune dysfunctions, and viral infections, highlighting its therapeutic potential.
These antibodies are typically raised in hosts like rabbits or mice, with validated specificity through knockout controls or siRNA knockdown. Researchers rely on ATP6AP1 antibodies to dissect V-ATPase-related mechanisms in cellular homeostasis and pathology, offering insights into novel drug targets for diseases linked to lysosomal dysfunction or aberrant proton transport.
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