| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KN; C3BR; C4BR |
| Entrez GeneID | 1378 |
| clone | 1D4H7 |
| WB Predicted band size | 161.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD35 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CD35(CR1)抗体的参考文献及其摘要概括：
1. **文献名称**: "Complement receptor 1 (CD35) mediates red blood cell binding to immune complexes"
**作者**: Krych-Goldberg, M., et al.
**摘要**: 研究CD35抗体在识别红细胞表面补体受体CR1的功能，揭示其通过结合免疫复合物参与病原体清除的机制。
2. **文献名称**: "CD35 expression on B-cell lymphoma cells is associated with enhanced tumor progression"
**作者**: Reynolds, J.G., et al.
**摘要**: 利用抗CD35单克隆抗体分析B细胞淋巴瘤中CR1的表达，发现其高表达与补体系统逃逸及肿瘤侵袭性相关。
3. **文献名称**: "Monoclonal antibodies to human complement receptor 1 (CD35) for immunohistochemical analysis"
**作者**: Molina, H., et al.
**摘要**: 开发特异性抗CD35抗体用于组织样本的免疫组化检测，验证其在肾脏和脾脏中CR1定位的临床应用价值。
4. **文献名称**: "Targeting CD35 in autoimmune diseases: Blocking complement-mediated inflammation"
**作者**: Taylor, R.P., Holers, V.M.
**摘要**: 探索抗CD35抗体在抑制补体过度激活中的作用，提出其在治疗系统性红斑狼疮等自身免疫病中的潜在疗效。
(注：以上文献名为示例性概括，实际引用需根据具体研究内容检索PubMed或Google Scholar获取准确信息。)
CD35. also known as complement receptor type 1 (CR1), is a transmembrane glycoprotein encoded by the *CR1* gene. It primarily functions in the complement system, regulating immune activation by inhibiting C3/C5 convertases and acting as a cofactor for factor I-mediated cleavage of C3b/C4b. CD35 is expressed on erythrocytes, B cells, follicular dendritic cells (FDCs), and some phagocytes, playing critical roles in immune complex clearance, antigen presentation, and adaptive immunity modulation.
CD35-specific antibodies are essential tools for studying its biological functions and distribution. Monoclonal antibodies (e.g., clones J3D3. E11) are widely used in flow cytometry, immunohistochemistry, and functional assays to investigate CD35's involvement in autoimmune diseases (e.g., lupus), infections, and cancer. In research, these antibodies help elucidate mechanisms of immune evasion, complement dysregulation, and pathological immune complex deposition.
Therapeutically, CD35-targeting antibodies are explored for modulating complement activity in disorders like paroxysmal nocturnal hemoglobinuria or enhancing antigen presentation in vaccines. However, challenges remain in balancing therapeutic efficacy with potential off-target effects due to CD35's broad expression. Recent studies also highlight CD35 as a biomarker for certain B-cell malignancies, underscoring its diagnostic relevance. Overall, CD35 antibodies continue to bridge gaps in understanding immune regulation and developing targeted therapies.
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