| WB | 1/500 - 1/2000 | Human, Monkey |
| IF | 咨询技术 | Human, Monkey |
| IHC | 1/200 - 1/1000 | Human, Monkey |
| ICC | 1/200 - 1/1000 | Human, Monkey |
| FCM | 1/200 - 1/400 | Human, Monkey |
| Elisa | 1/10000 | Human, Monkey |
| Entrez GeneID | 7408 |
| clone | 6B10A1 |
| WB Predicted band size | 39.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Monkey |
| Immunogen | Purified recombinant fragment of human VASP (AA: 1-380) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于VASP抗体的参考文献及其简要摘要：
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1. **文献名称**: *"The role of VASP in cell migration and focal adhesion dynamics"*
**作者**: Schwarz et al.
**摘要**: 该研究利用VASP特异性抗体，通过免疫荧光和Western blot分析，揭示了VASP蛋白在调控细胞迁移和黏着斑形成中的关键作用，表明其磷酸化状态影响细胞骨架重组。
2. **文献名称**: *"VASP phosphorylation as a biomarker of endothelial dysfunction in cardiovascular disease"*
**作者**: Oelze et al.
**摘要**: 通过检测患者血液中磷酸化VASP(使用VASP抗体及磷酸化特异性抗体)，研究发现VASP的Ser239磷酸化水平与血管内皮功能损伤显著相关，可作为心血管疾病的潜在诊断标志物。
3. **文献名称**: *"VASP regulates platelet activation and immune thrombocytopenia"*
**作者**: Li et al.
**摘要**: 该文献使用VASP抗体研究血小板中VASP的表达与功能，发现VASP缺失会增强血小板活化，提示其在免疫性血小板减少症中的调控机制，为治疗策略提供新靶点。
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以上文献涵盖了VASP抗体在基础细胞生物学、临床诊断及血液疾病研究中的应用，均通过抗体检测VASP表达或修饰状态以探索其功能。
Vasodilator-Stimulated Phosphoprotein (VASP) is a key regulatory protein involved in actin cytoskeleton dynamics, cell motility, adhesion, and proliferation. It belongs to the Ena/VASP family, which interacts with profilin and other actin-binding proteins to modulate filament elongation and bundling. VASP contains conserved domains, including the EVH1 (Enabling/VASP homology 1) domain for protein-protein interactions and the EVH2 domain for actin binding. Its activity is tightly regulated by phosphorylation, particularly at Ser157. Ser239. and Thr278. mediated by cyclic nucleotide-dependent kinases (e.g., PKA, PKG) in response to cellular signals like cAMP/cGMP.
VASP antibodies are essential tools for studying its expression, localization, and post-translational modifications. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to explore VASP's role in cellular processes. For instance, these antibodies help elucidate how VASP phosphorylation influences endothelial barrier function, platelet activation, or cancer cell migration. Dysregulation of VASP is linked to cardiovascular diseases, metastatic cancers, and immune disorders, making it a potential therapeutic target. Researchers also employ VASP antibodies to assess the efficacy of pharmacological agents targeting cAMP/PKG pathways. Specific monoclonal or polyclonal antibodies are validated for detecting distinct phosphorylation states or total protein levels, ensuring precision in mechanistic studies. Overall, VASP antibodies serve as critical reagents for advancing understanding of cytoskeletal dynamics in health and disease.
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