关键信息
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基因名
Caspase-8/CASP8 subunit p18
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简介
The Caspase-8/CASP8 subunit p18 protein is a key thiol protease that acts as a molecular switch in programmed cell death processes, including apoptosis, necroptosis, and pyroptosis. It is essential for preventing tissue damage by inducing exogenous apoptosis by cleaving and activating effector caspases. Caspase-8/CASP8 subunit p18 Protein, Human (His) is the recombinant human-derived Caspase-8/CASP8 subunit p18 protein, expressed by E. coli , with N-6*His labeled tag.
- 应用
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别名
ALPS2B; CASP-8; ICE-like apoptotic protease 5
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14790
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表达区间
S217-D374
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蛋白长度
Full Length of CASP8 subunit p18
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分子量
21.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Caspase-8 (CASP8) is a pivotal cysteine protease that plays a crucial role in apoptosis, inflammation, and immune response. It is a key initiator caspase, primarily involved in the extrinsic apoptotic signaling pathway triggered by death receptors. The active form of Caspase-8 exists as a heterotetramer composed of two large subunits (p18) and two small subunits (p10). Research focusing on the recombinant p18 subunit of Caspase-8 is essential for understanding its structure-function relationship, regulatory mechanisms, and potential therapeutic applications. The recombinant p18 protein can be utilized to elucidate its role in apoptotic pathways and to investigate how mutations or dysregulation in its expression contribute to various diseases, including cancer and autoimmune disorders. Moreover, studying the p18 subunit offers insights into the design of specific inhibitors that could modulate its activity for therapeutic purposes. Given the importance of Caspase-8 in both cell death and survival pathways, advancements in recombinant protein technologies provide a valuable platform for drug discovery and the development of novel treatment strategies targeting apoptotic dysregulation.
