关键信息
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基因名
Apolipoprotein E/APOE2
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简介
Apolipoprotein E (APOE) plays a key role in lipoprotein-mediated lipid transport and is a core component in the production, transformation, and clearance of plasma lipoproteins. As an amphipathic molecule, APOE binds to various lipoprotein particles, including chylomicrons, chylomicron remnants, VLDL, and IDL, favoring HDL. Apolipoprotein E/APOE Protein, Human (R154S, R176C, HEK293, C-His) is the recombinant human-derived Apolipoprotein E/APOE protein, expressed by HEK293 , with C-6*His labeled tag and R154S, R176C mutation.
- 应用
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生物活性
Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED50 this effect is ≤8.753 ng/mL. corresponding to a specific activity is ≥1.14×105 units/mg. Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED50 for this effect is 8.753 ng/mL.corresponding to a specific activity is 1.14×105units/mg.
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别名
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02649
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表达区间
K19-H317, R154S, R176C
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氨基酸序列
KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVSLASHLRKLRKRLLRDADDLQKCLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH
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蛋白长度
Full Length of Mature Protein
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分子量
33-42 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Apolipoprotein E (APOE) is a key protein involved in lipid metabolism and is known for its crucial role in cardiovascular health and neurobiology. Among its isoforms—APOE2, APOE3, and APOE4—APOE2 has garnered attention for its potential protective effects against Alzheimer's disease. Research indicates that individuals carrying the APOE2 allele exhibit a lower risk of developing this neurodegenerative condition compared to those with the more prevalent APOE4 allele, which is associated with increased risk. Understanding the structure, function, and interactions of APOE2 can provide insights into its protective mechanisms, which may offer therapeutic targets for Alzheimer's disease and other disorders. Recombinant protein studies of APOE2 facilitate the exploration of its biological activities, including its role in lipid transport and neuronal repair processes. Moreover, such recombinant proteins can be utilized in drug development, biomarker discovery, and the creation of novel therapeutic interventions aimed at enhancing cognitive function or preventing neurodegeneration. The multifaceted nature of APOE2 necessitates ongoing research to unravel its specific biological pathways, making it a focal point for studies in neurobiology, genetics, and protein science. Hence, recombinant APOE2 protein research not only deepens our understanding of lipid metabolism and neuroprotection but also raises hopes for innovations in treating Alzheimer’s disease and other lipid-related disorders.
