| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324；E-cadherin；E cadherin |
| Entrez GeneID | 999 |
| clone | 6G2B3 |
| WB Predicted band size | 97.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CDH1 (AA:extra(155-354)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于CDH1抗体的3篇代表性文献及其摘要(示例参考格式)：
1. **文献名称**：*Loss of E-cadherin expression in gastric cancer: Role of CDH1 gene mutation and immunohistochemical analysis*
**作者**：Becker KF, et al.
**摘要**：本研究通过免疫组化(使用CDH1特异性抗体)和基因测序，发现胃癌中E-cadherin表达缺失与CDH1基因突变及肿瘤转移显著相关，提示其作为预后标志物的潜力。
2. **文献名称**：*E-cadherin antibody-based magnetic cell sorting for isolating breast cancer stem cells*
**作者**：Ponti D, et al.
**摘要**：利用CDH1抗体结合磁性分选技术，成功分离出乳腺癌干细胞亚群，证实E-cadherin低表达与肿瘤干细胞特性及化疗耐药性相关。
3. **文献名称**：*Validation of CDH1 antibody specificity in hereditary diffuse gastric cancer models*
**作者**：Brooks-Wilson AR, et al.
**摘要**：通过Western blot和免疫荧光验证多种商用CDH1抗体的特异性，发现克隆HECD-1在检测遗传性弥漫型胃癌模型中的E-cadherin截短蛋白效果最佳。
---
*注：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索获取。建议使用关键词“CDH1 antibody”、“E-cadherin immunohistochemistry”或结合具体研究领域(如癌症亚型)进行精准查询。*
The CDH1 antibody targets E-cadherin, a calcium-dependent transmembrane glycoprotein encoded by the *CDH1* gene. E-cadherin plays a critical role in maintaining epithelial cell-cell adhesion and tissue integrity through homophilic interactions. It is a key component of adherens junctions, linking to the actin cytoskeleton via catenins (e.g., β-catenin), and is essential for embryonic development, epithelial polarization, and wound healing. Loss or dysfunction of E-cadherin is associated with epithelial-mesenchymal transition (EMT), a process driving cancer metastasis and invasiveness, particularly in carcinomas like breast, gastric, and colorectal cancers. CDH1 mutations or epigenetic silencing are hallmarks of aggressive tumors and correlate with poor prognosis.
CDH1 antibodies are widely used in research and diagnostics to assess E-cadherin expression levels in tissues or cell lines. In immunohistochemistry (IHC), they help identify epithelial differentiation in tumors or evaluate EMT status. Reduced E-cadherin staining often indicates metastatic potential. These antibodies also aid in studying hereditary diffuse gastric cancer (HDGC), where germline *CDH1* mutations predispose individuals to malignancy. Commercially available clones (e.g., 36/E-cadherin, NCH-38) vary in specificity for intracellular vs. extracellular domains, requiring validation for experimental contexts. Challenges include cross-reactivity with other cadherins and sensitivity to tissue fixation methods. Overall, CDH1 antibodies remain vital tools for understanding cell adhesion biology and cancer progression.
×
