| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | POMC; LPH; MSH; NPP; POC; CLIP; OBAIRH |
| Entrez GeneID | 5443 |
| clone | 2B4A1 |
| WB Predicted band size | 29.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ACTH expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是与ACTH抗体相关的3篇参考文献示例(注：文献信息为示例性内容，非真实文献)：
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1. **文献名称**：*Development of a High-Sensitivity ELISA for ACTH Measurement Using Monoclonal Antibodies*
**作者**：Smith J, et al.
**摘要**：本研究描述了一种基于单克隆ACTH抗体的新型ELISA检测方法，提高了血浆中低浓度ACTH的检测灵敏度，适用于库欣综合征的早期诊断。
2. **文献名称**：*Characterization of ACTH-Specific Antibodies and Their Cross-Reactivity with Related Peptides*
**作者**：Johnson R, et al.
**摘要**：通过免疫印迹和竞争结合实验，分析了多种ACTH抗体的特异性，发现部分抗体与α-MSH等类似肽存在交叉反应，提示临床检测中需谨慎选择抗体。
3. **文献名称**：*ACTH Antibody-Based Immunoassays in the Diagnosis of Adrenal Disorders*
**作者**：Brown K, et al.
**摘要**：比较了不同ACTH抗体免疫检测法在肾上腺功能不全和库欣病中的表现，证实单克隆抗体检测在区分异位ACTH分泌方面具有更高准确性。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“ACTH antibody immunoassay”或“ACTH antibody clinical application”。
Adrenocorticotropic hormone (ACTH), a peptide hormone produced by the anterior pituitary gland, regulates cortisol secretion from the adrenal cortex. ACTH antibodies are essential tools in research and diagnostics for detecting and quantifying ACTH levels. These antibodies are typically developed using animal immunization with synthetic ACTH fragments or recombinant proteins, generating polyclonal or monoclonal variants. Monoclonal antibodies offer high specificity by targeting distinct epitopes, while polyclonal antibodies may detect multiple ACTH forms, including precursor molecules like pro-opiomelanocortin (POMC).
In clinical settings, ACTH antibodies are pivotal for immunoassays (e.g., ELISA, RIA) to diagnose disorders of the hypothalamic-pituitary-adrenal (HPA) axis. They help differentiate conditions such as Cushing's syndrome (excess cortisol), Addison's disease (adrenal insufficiency), and ectopic ACTH-secreting tumors. Additionally, immunohistochemistry using ACTH antibodies aids in localizing hormone production in pituitary adenomas or neuroendocrine tumors.
Challenges include cross-reactivity with ACTH-related peptides and matrix interference in biological samples. Advances in antibody engineering, such as epitope mapping and affinity purification, have improved assay accuracy. Research applications extend to studying stress responses, circadian rhythm regulation, and HPA axis dysfunctions in metabolic or psychiatric disorders. Validated ACTH antibodies remain critical for both diagnostic precision and mechanistic investigations in endocrinology.
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