| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 947 |
| clone | 6C7G7 |
| WB Predicted band size | 40.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD34 (AA: extra 32-290) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与CD34抗体相关的研究文献及其简要摘要：
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1. **"Antigenic analysis of hematopoiesis: a hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells"**
*Civin CI, Strauss LC, Fackler MJ*
该研究首次报道了针对CD34抗原的单克隆抗体的开发，证实CD34在人类造血干细胞和祖细胞表面特异性表达，为后续干细胞分选技术奠定基础。
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2. **"CD34+ marrow cells analyzed for long-term culture initiating cells (LTC-ICs) and myelopoiesis in vitro"**
*Simmons PJ, Torok-Storb B*
研究利用CD34抗体分选骨髓细胞，证明CD34阳性细胞具有长期培养启动能力，并能在体外支持髓系造血，揭示了其在造血重建中的关键作用。
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3. **"Isolation of putative progenitor endothelial cells for angiogenesis"**
*Asahara T, Murohara T, Sullivan A, et al.*
通过CD34抗体识别并分离循环中的内皮祖细胞，证实这些细胞参与血管新生和缺血组织修复，扩展了CD34在血管生物学领域的应用价值。
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(注：以上文献为领域内经典研究示例，实际引用时需核对最新版本或数据库具体条目。)
CD34 antibodies are essential tools in biomedical research and clinical diagnostics, targeting the CD34 glycoprotein expressed on the surface of hematopoietic progenitor cells, vascular endothelial cells, and certain stromal cells. First identified in the late 1980s, CD34 is a 110–120 kDa transmembrane protein involved in cell adhesion, migration, and signaling. Its expression is closely associated with primitive hematopoietic stem cells (HSCs), making it a key marker for isolating these cells in bone marrow transplantation and regenerative medicine.
In clinical practice, CD34 antibodies are widely used in flow cytometry and immunohistochemistry to quantify and characterize HSCs, assess engraftment potential, and diagnose hematologic malignancies such as acute myeloid leukemia. They also aid in studying vascular biology, as CD34 is expressed in vascular endothelial cells during angiogenesis.
Despite its utility, CD34's expression isn't exclusive to HSCs; it can appear in some tumors and non-hematopoietic tissues, necessitating complementary markers for specificity. Antibodies like QBEnd10 (a common anti-CD34 clone) are standardized in protocols for consistency. Ongoing research explores CD34's functional roles beyond cell adhesion, including its potential in tissue repair and immune regulation.
Overall, CD34 antibodies remain pivotal in advancing stem cell therapies, cancer diagnostics, and understanding vascular and immune system dynamics.
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