| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BCL2L4 |
| Entrez GeneID | 581 |
| clone | 7D8B2 |
| WB Predicted band size | 21.2kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human BAX (AA:(13-160)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与BAX抗体相关的代表性文献示例(注：内容为模拟概括，实际文献请通过学术数据库查询)：
1. **文献名称**：A novel monoclonal antibody specifically recognizes the active conformation of BAX protein
**作者**：Smith J, et al.
**摘要**：本研究开发了一种新型单克隆抗体，可特异性识别BAX蛋白的活性构象(寡聚化状态)。通过免疫印迹和免疫荧光验证，该抗体能有效区分凋亡过程中BAX的激活状态，为研究细胞凋亡动态机制提供了关键工具。
2. **文献名称**：Differential expression of BAX in colorectal cancer detected by immunohistochemistry
**作者**：Chen L, et al.
**摘要**：利用商业化BAX抗体(克隆号D2E11)对结直肠癌组织进行免疫组化分析，发现BAX表达水平与肿瘤分期和化疗敏感性显著相关，提示其可作为预后生物标志物的潜在价值。
3. **文献名称**：Validation of BAX antibody specificity in flow cytometry-based apoptosis assays
**作者**：Rodriguez M, et al.
**摘要**：系统评估了6种市售BAX抗体在流式细胞术中的特异性，发现克隆号E63在不同细胞系中表现出最优的信噪比。研究强调抗体验证对凋亡定量分析的重要性，并提供了标准化实验方案。
4. **文献名称**：BAX oligomerization in mitochondrial apoptosis: Insights from conformational-specific antibodies
**作者**：Walensky LD, et al.
**摘要**：通过构象特异性抗体揭示BAX在线粒体外膜寡聚化的动态过程，证实其与细胞色素C释放的直接关联，为靶向BAX的癌症治疗策略提供了结构生物学依据。
BAX (Bcl-2-associated X protein) is a key pro-apoptotic member of the Bcl-2 protein family, which regulates programmed cell death (apoptosis). In response to cellular stress or DNA damage, BAX undergoes conformational activation, translocates to the mitochondrial outer membrane, and forms oligomers that permeabilize mitochondria, triggering cytochrome c release and caspase cascade activation. Dysregulation of BAX is implicated in diseases such as cancer (where reduced BAX expression may promote survival) and neurodegenerative disorders (where excessive apoptosis may occur).
BAX antibodies are essential tools for detecting BAX expression, localization, and activation status in research. These antibodies, often monoclonal or polyclonal, target specific epitopes (e.g., N-terminal, C-terminal, or conformation-specific regions) and are used in techniques like Western blotting, immunohistochemistry, and flow cytometry. They help elucidate BAX’s role in apoptosis mechanisms, drug response studies, and disease biomarkers. For example, BAX antibodies can distinguish between inactive monomeric and active oligomeric forms, providing insights into apoptotic pathways. Validation of BAX antibodies requires attention to specificity, cross-reactivity, and application compatibility, ensuring accurate interpretation of BAX’s functional states in physiological and pathological contexts.
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