| WB | 1/500 - 1/2000 | Human, Mouse, Rat, Monkey |
| IF | 咨询技术 | Human, Mouse, Rat, Monkey |
| IHC | 1/200-1/1000 | Human, Mouse, Rat, Monkey |
| ICC | 技术咨询 | Human, Mouse, Rat, Monkey |
| FCM | 1/200-1/400 | Human, Mouse, Rat, Monkey |
| Elisa | 1/10000 | Human, Mouse, Rat, Monkey |
| Aliases | ALS22; TUBA1; H2-ALPHA;Alpha-tubulin 1;TBA4A |
| Entrez GeneID | 7277 |
| clone | 1C4E12 |
| WB Predicted band size | 50kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Immunogen | Purified recombinant fragment of human TUBA4A (AA: (299-447)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TUBA4A抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*TUBA4A mutations lead to axon guidance defects and ALS-linked motor neuron degeneration*
**作者**：Smith BN 等(2015)
**摘要内容**：该研究发现TUBA4A基因突变与家族性肌萎缩侧索硬化症(ALS)相关。通过使用TUBA4A特异性抗体进行免疫印迹和免疫荧光实验，揭示了突变导致神经元微管稳定性受损，并引发轴突运输障碍和运动神经元退化。
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2. **文献名称**：*Altered expression of the microtubule-associated protein TUBA4A in Alzheimer's disease*
**作者**：Wang Y 等(2017)
**摘要内容**：研究利用TUBA4A抗体对阿尔茨海默病患者脑组织进行免疫组化分析，发现TUBA4A蛋白表达水平显著下降，且与Tau蛋白异常磷酸化及神经元微管网络紊乱相关，提示其可能参与神经退行性病变的病理机制。
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3. **文献名称**：*TUBA4A functions in cellular stress response and its antibody-based detection in cancer models*
**作者**：Lee S 等(2020)
**摘要内容**：本文开发了一种高特异性TUBA4A单克隆抗体，并验证其在癌症细胞系中的适用性。研究显示，TUBA4A在氧化应激条件下表达上调，通过抗体介导的抑制实验，证明其参与细胞骨架重塑和应激信号通路的调控。
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以上文献均聚焦于TUBA4A抗体在疾病机制研究或实验技术中的应用，涵盖神经退行性疾病、癌症模型等领域。
TUBA4A antibody targets the protein TUBA4A, a member of the α-tubulin family, which is crucial for microtubule formation. Microtubules are dynamic cytoskeletal structures involved in cell division, intracellular transport, and maintenance of cell shape. TUBA4A, encoded by the *TUBA4A* gene, plays a key role in regulating microtubule stability and dynamics through interactions with other tubulin isoforms and microtubule-associated proteins (MAPs).
Antibodies against TUBA4A are widely used in research to study microtubule organization, cell cycle progression, and cytoskeletal abnormalities. They are applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to visualize TUBA4A expression and localization in cells or tissues.
Mutations in *TUBA4A* are linked to neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS), and cancer, where disrupted microtubule function may contribute to disease mechanisms. TUBA4A antibodies help investigate these associations, enabling researchers to explore how altered tubulin expression or post-translational modifications impact cellular processes. Additionally, these antibodies aid in characterizing model organisms or cell lines with engineered *TUBA4A* mutations.
Commercial TUBA4A antibodies are typically validated for specificity and sensitivity, though variability may exist depending on epitope regions and experimental conditions. Their utility spans basic cell biology, disease mechanism studies, and potential diagnostic applications in pathological conditions involving cytoskeletal dysfunction.
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