| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CDX3; CDX-3; CDX2/AS |
| Entrez GeneID | 1045 |
| clone | 5C11H10 |
| WB Predicted band size | 33.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CDX2 (AA: 1-180) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CDX2抗体的3篇参考文献的简要整理：
1. **"CDX2: A Highly Sensitive and Specific Marker of Colorectal Adenocarcinoma"**
- **作者**: Werling RW, et al.
- **摘要**: 该研究验证了CDX2抗体在区分结直肠腺癌与其他上皮性肿瘤中的高敏感性和特异性，表明其在病理诊断中作为肠道分化标志物的价值。
2. **"CDX2 Immunostaining as a Marker of Intestinal Differentiation in Gastric Carcinomas"**
- **作者**: Bai Y, et al.
- **摘要**: 文章探讨CDX2在胃癌中的表达模式，发现其与肠上皮化生和肠型胃癌密切相关，提示CDX2抗体可用于胃癌分型和预后评估。
3. **"CDX2 in Ovarian Mucinous Tumors: A Clinicopathological Study"**
- **作者**: Moskowitz R, et al.
- **摘要**: 通过免疫组化分析，研究发现CDX2抗体可有效区分卵巢原发黏液性肿瘤与转移性结直肠癌，支持其在妇科病理鉴别诊断中的应用。
4. **"Utility of CDX2 in the Diagnosis of Metastatic Carcinoma of Unknown Primary"**
- **作者**: Kaimaktchiev V, et al.
- **摘要**: 该研究评估CDX2抗体在追踪转移性肿瘤原发部位中的作用，强调其在消化道来源肿瘤(如结直肠癌)鉴别中的高特异性。
(注：以上文献标题和内容为示例性概括，具体研究需根据实际数据库检索验证。)
The CDX2 antibody is a widely used diagnostic tool in pathology and research for identifying the presence of the caudal-type homeobox transcription factor 2 (CDX2), a protein critical in intestinal development and differentiation. CDX2. encoded by the *CDX2* gene, regulates the expression of genes involved in maintaining intestinal epithelial cell identity, proliferation, and apoptosis. It is primarily expressed in the nuclei of normal intestinal epithelial cells, making it a sensitive marker for intestinal lineage.
In diagnostic pathology, CDX2 immunohistochemistry (IHC) is pivotal for determining the origin of adenocarcinomas, particularly in metastatic or poorly differentiated tumors. Strong nuclear CDX2 expression is strongly associated with colorectal carcinomas (positive in >95% of cases), aiding in distinguishing them from other gastrointestinal or non-gastrointestinal malignancies. However, CDX2 is not entirely specific; it may also be expressed in gastric, pancreaticobiliary, ovarian mucinous, and some neuroendocrine tumors.
Research applications of CDX2 antibodies include studying intestinal metaplasia, Barrett’s esophagus, and developmental biology. Reduced CDX2 expression correlates with poor differentiation, aggressive behavior, and worse prognosis in colorectal cancer. Additionally, CDX2 loss is observed in certain hereditary polyposis syndromes. Commercially available clones (e.g., CDX2-88) are validated for IHC, ensuring reliability in both diagnostic and experimental settings. Its role in maintaining intestinal homeostasis and its dysregulation in neoplasia continue to make CDX2 a key focus in translational research.
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