| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22 |
| Entrez GeneID | 2950 |
| clone | 1A7B5 |
| WB Predicted band size | 23kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GSTP1 (AA: 2-210) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GSTP1抗体的3-4篇参考文献及其简要摘要：
1. **文献名称**: "GSTP1 CpG island hypermethylation as a molecular biomarker for prostate cancer"
**作者**: Nakayama, M. et al.
**摘要**: 该研究利用GSTP1抗体检测前列腺癌组织中GSTP1蛋白的表达缺失，发现其与CpG岛甲基化相关，提出GSTP1作为前列腺癌早期诊断的表观遗传生物标志物。
2. **文献名称**: "Human glutathione S-transferase P1-1: an enzyme in cancer chemoprevention and therapy"
**作者**: Tew, K.D. & Ronai, Z.
**摘要**: 文章探讨了GSTP1在肿瘤耐药性中的作用，通过抗体检测其在多种癌细胞中的过表达，揭示了GSTP1通过代谢解毒化疗药物影响治疗效果。
3. **文献名称**: "Immunohistochemical analysis of glutathione S-transferase Pi expression in human breast cancer"
**作者**: Townsend, A.J. & Tew, K.D.
**摘要**: 使用GSTP1特异性抗体对乳腺癌组织进行免疫组化分析，发现GSTP1表达水平与肿瘤恶性程度及患者预后呈负相关，提示其作为预后标志物的潜力。
4. **文献名称**: "Antibody specificity in human glutathione S-transferase detection: comparative analysis of isoforms"
**作者**: Ali-Osman, F. et al.
**摘要**: 研究比较了不同GSTP1抗体的特异性，验证了其在Western blot和免疫荧光中的适用性，强调抗体选择对准确检测GSTP1异构体的重要性。
以上文献涵盖了GSTP1抗体在癌症机制、诊断及实验方法中的应用。
The glutathione S-transferase P1 (GSTP1) antibody is a crucial tool for studying the GSTP1 protein, a member of the glutathione S-transferase (GST) family involved in cellular detoxification and oxidative stress response. GSTP1 conjugates glutathione to electrophilic substrates, neutralizing toxins, carcinogens, and reactive oxygen species. It is highly expressed in normal tissues, including the liver, prostate, and lung, but frequently dysregulated in cancers. Notably, GSTP1 silencing via promoter hypermethylation is a hallmark of prostate cancer, making it a potential diagnostic biomarker.
GSTP1 antibodies are widely used in research to detect protein expression and localization via techniques like Western blot, immunohistochemistry (IHC), and immunofluorescence (IF). They help elucidate GSTP1's role in drug resistance, as its overexpression in tumors may impair chemotherapeutic efficacy by detoxifying anticancer agents. Additionally, these antibodies aid in exploring GSTP1's non-catalytic functions, such as modulating stress signaling pathways (e.g., JNK and MAPK) through protein-protein interactions.
Commercial GSTP1 antibodies are typically raised against specific epitopes, with validation in various species and applications. Researchers must verify antibody specificity using controls like GSTP1-knockout samples, as cross-reactivity with other GST isoforms (e.g., GSTA or GSTM) can occur. Understanding GSTP1's dual roles in cytoprotection and oncogenesis continues to drive interest in therapeutic targeting, with its antibody remaining indispensable for mechanistic and translational studies.
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