| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CD56; NCAM; MSK39 |
| Entrez GeneID | 4684 |
| clone | 2C5E7 |
| WB Predicted band size | 94.6kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NCAM1 (AA: extra(568-708)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于NCAM1抗体的参考文献及其摘要概括：
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1. **文献名称**: *"NCAM1 Antibody Characterization in Neural Development Studies"*
**作者**: Müller, T. et al.
**摘要**: 本研究验证了一种高特异性NCAM1单克隆抗体在斑马鱼和小鼠神经组织中的应用，证明其可通过免疫荧光标记发育中的神经元迁移路径，并揭示NCAM1在轴突导向中的动态表达模式。
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2. **文献名称**: *"Targeting NCAM1 with Humanized Antibodies Suppresses Metastasis in Colorectal Cancer"*
**作者**: Chen, L. & Wang, H.
**摘要**: 文章开发了一种人源化NCAM1抗体(huNCAM1-mAb)，通过阻断NCAM1介导的细胞黏附信号通路，显著抑制结直肠癌小鼠模型的肿瘤转移，提示其作为新型治疗策略的潜力。
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3. **文献名称**: *"A Comparative Study of Commercial Anti-NCAM1 Antibodies for Flow Cytometry"*
**作者**: Rossi, G. et al.
**摘要**: 系统性评估了6种市售NCAM1抗体的性能，发现克隆号5B8的抗体在流式细胞术中特异性最佳，适用于白血病细胞表面NCAM1的精准定量，为临床样本检测提供优化方案。
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4. **文献名称**: *"NCAM1 Antibody-Based PET Imaging for Neurodegenerative Disease Diagnosis"*
**作者**: Kim, S. et al.
**摘要**: 研究利用放射性标记的NCAM1抗体(^89Zr-DOTA-anti-NCAM1)进行PET成像，成功在小鼠阿尔茨海默病模型中检测到突触丢失区域的NCAM1表达变化，提出无创诊断神经退行性疾病的新方法。
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每篇文献均围绕NCAM1抗体的开发、验证或应用展开，涵盖基础研究、癌症治疗、诊断工具开发等方向。
**Background of NCAM1 Antibodies**
NCAM1 (Neural Cell Adhesion Molecule 1), also known as CD56. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It plays critical roles in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and immune regulation. NCAM1 is widely expressed in the nervous system, particularly in neurons, glial cells, and neural stem cells, but is also found in certain immune cells (e.g., natural killer cells) and tumors (e.g., neuroblastoma, small cell lung cancer).
Antibodies targeting NCAM1 are essential tools for studying its biological functions and pathological roles. They enable detection of NCAM1 expression in tissues or cells via techniques like immunohistochemistry, flow cytometry, and Western blot. These antibodies can distinguish between NCAM1 isoforms (e.g., NCAM-120. NCAM-140. NCAM-180) arising from alternative splicing, as well as post-translational modifications like polysialylation, which modulates cell adhesion.
In research, NCAM1 antibodies help investigate neural development, neurodegenerative diseases (e.g., Alzheimer’s), and cancer metastasis. Clinically, they aid in diagnosing NCAM1-positive tumors and monitoring disease progression. Therapeutic applications are emerging, with antibody-drug conjugates (ADCs) or CAR-T therapies targeting NCAM1 in certain malignancies. However, challenges remain, including cross-reactivity with other proteins and variability in isoform detection.
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